Positional relationship between the gamete fusion site and the first division plane in the rice zygote.
Positional relationship between the gamete fusion site and the first division plane in the rice zygote.
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DOI：
10.1093/jxb/erq131
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发表时间：
2010-06
影响因子：
6.9
通讯作者：
Okamoto T
中科院分区：
文献类型：
--
作者：
Nakajima K;Uchiumi T;Okamoto T
在被子植物中，受精卵通常分裂成由顶端细胞和基细胞组成的两细胞原胚，它们拥有不同的细胞命运。受精卵的第一次分裂可能是形成原胚顶-基轴的一个步骤。配子融合激活卵子，而合子上的配子融合部位被报道为动植物随后的合子发育和/或胚胎模式提供可能的线索。在这项研究中，水稻受精卵上的配子融合部位被标记为水稻卵细胞与荧光染色的精子细胞的体外受精。利用融合部位标记的受精卵的固定培养，监测配子融合部位与受精卵分裂形成的分裂平面之间的位置关系，直到二细胞原胚期。结果表明，两细胞原胚上存在配子融合部位，与第一分裂面的位置无关，水稻受精卵上的配子融合部位不是合子分裂面定位的决定因素。
In angiosperms, a zygote generally divides into a two-celled proembryo consisting of an apical and a basal cell that possess different cell fates. This first division of the zygote is a putative step in the formation of the apical–basal axis of the proembryo. The gamete fusion activates the egg, and the gamete fusion site on the zygote has been reported to provide a possible cue for subsequent zygotic development and/or embryonic patterning in animals and plants. In this study, the gamete fusion site on the rice zygote was labelled by in vitro fertilization of a rice egg cell with a fluorescence-stained sperm cell. The positional relationship between the gamete fusion site and the division plane formed by zygotic cleavage was monitored using a fixed culture of the fusion site-labelled zygote until the two-celled proembryo stage. The results indicate that gamete fusion sites exist on two-celled proembryos with no relation to the position of the first division plane, and that the gamete fusion site on the rice zygote does not function as a determinant for positioning the zygote division plane.