Levels of insecticide resistance and resistance mechanisms in Aedes aegypti from some Latin American countries
Levels of insecticide resistance and resistance mechanisms in Aedes aegypti from some Latin American countries
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DOI：
10.2987/5588.1
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发表时间：
2007-12-01
影响因子：
1
通讯作者：
Fernandez, Ditter
中科院分区：
文献类型：
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作者：
Rodriguez, Maria M.;Bisset, Juan A.;Fernandez, Ditter
在实验室条件下评估了 8 种拉丁美洲埃及伊蚊菌株对 6 种有机磷酸酯（替美磷、马拉硫磷、倍硫磷、甲基嘧啶磷、杀螟硫磷和毒死蜱）和 4 种拟除虫菊酯（溴氰菊酯、高效氯氟氰菊酯、高效氯氰菊酯和氟氯氰菊酯）的耐药性。在幼虫生物测定中，大多数菌株对替美磷耐药性较高（耐药率[RR50]，>= 10X），但尼加拉瓜和委内瑞拉菌株除外，它们表现出中等耐药性（RR50，在5至10X之间）。大多数菌株对马拉硫磷、倍硫磷和杀螟松敏感。然而，大多数菌株对甲基嘧啶磷的耐药性从中度到高度不等。来自哈瓦那市的幼虫对所测试的 3 种拟除虫菊酯具有抗药性，对氟氯氰菊酯具有中度抗药性。古巴圣地亚哥菌株对溴氰菊酯表现出高抗性，对其他拟除虫菊酯（高效氯氟氰菊酯、高效氯氰菊酯和氟氯氰菊酯）表现出中度抗性。除牙买加和哥斯达黎加菌株对氟氯氰菊酯表现出中度抗药性，以及秘鲁和委内瑞拉菌株对溴氰菊酯表现出抗药性外，其余菌株均对拟除虫菊酯敏感。成虫生物测定表明，所有菌株都对二氯二苯基三氯乙烷和大多数评估的拟除虫菊酯具有抗性。增效剂S,S,S,-三丁基三硫代磷酸酯和胡椒腈丁醚的使用表明，酯酶和单加氧酶在一些菌株对替美磷、甲基嘧啶磷和毒死蜱的抗性中发挥着重要作用。生化测试显示高频率的酯酶和谷胱甘肽-S-转移酶活性；然而，乙酰胆碱酯酶机制改变的频率较低。聚丙烯酰胺电泳凝胶检测到存在名为 Est-A4 的强条带。伊蚊的杀虫剂抗性。埃及伊蚊是防治工作面临的一个严重问题，建议采用综合防治策略来帮助预防或延缓幼虫对替美磷的抗药性和成虫对拟除虫菊酯的抗药性。
Eight Latin American strains of Aedes aegypti were evaluated for resistance to 6 organophosphates (temephos, malathion, fenthion, pirimiphos-methyl, fenitrothion, and chlorpirifos) and 4 pyrethroids (deltamethrin, lambdacyhalothrin, betacypermethrin, and cyfluthrin) under laboratory conditions. In larval bioassays, temephos resistance was high (resistance ratio [RR50], >= 10X) in the majority of the strains, except for the Nicaragua and Venezuela strains, which showed moderate resistance (RR50, between 5 and 10X). The majority of the strains were susceptible to malathion, fenthion, and fenitrothion. However, resistance to pirimiphos-methyl ranged from moderate to high in most of the strains. Larvae from Havana City were resistant to 3 of the pyrethroids tested and moderately resistant to cyfluthrin. The Santiago de Cuba strain showed high resistance to deltamethrin and moderate resistance to the other pyrethroids (lambdacyhalothrin, betacypermethrin, and cyfluthrin). The rest of the strains were susceptible to pyrethroids, except for the Jamaica and Costa Rica strains, which showed moderate resistance to cyfluthrin, and Peru and Venezuela, which showed resistance to deltamethrin. Adult bioassays showed that all the strains were resistant to dichlorodiphenyl-trichloroethane and to the majority of pyrethroids evaluated. The use of the synergists S,S,S,-tributyl phosphorotrithioate and piperonil butoxide showed that esterase and monooxygenases played an important role in the temephos, pirimiphos-methyl, and chlorpirifos resistance in some strains. Biochemical tests showed high frequencies of esterase and glutathione-S-transferase activity; however, the frequency of altered acetylcholinesterase mechanism was low. The polyacrylamide electrophoresis gel detected the presence of a strong band called Est-A4. Insecticide resistance in Ae. aegypti is a serious problem facing control operations, and integrated control strategies are recommended to help prevent or delay the temephos resistance in larvae and pyrethroids resistance in adults.