In vivo oxygen detection using exogenous hemoglobin as a contrast agent in magnetic resonance microscopy
In vivo oxygen detection using exogenous hemoglobin as a contrast agent in magnetic resonance microscopy
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DOI：
10.1002/mrm.10405
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发表时间：
2003-04-01
影响因子：
3.3
通讯作者：
Jasanoff, A
中科院分区：
文献类型：
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作者：
Sun, PZ;Schoening, ZB;Jasanoff, A
在这项工作中，我们证明外源分子血红蛋白 (Hb) 是体内磁共振 (MR) 显微镜研究中相对局部氧张力的有效指标。这种方法比其他 MRI 血氧测定方法更灵敏；它可以在更高的分辨率下使用，并且用于没有血氧水平依赖性 (BOLD) 效应的样本。通过对果蝇的注射研究，我们表明 Hb 可以渗透相对致密的神经组织，并且不会明显破坏生理机能。当人为地将外部 O2 水平从 0% 调节到 21% 时，注射 Hb 的果蝇显示出信号强度的巨大变化 (40-50%)。在 T2 加权成像实验中检测到外源性 Hb 产生的氧依赖性对比度变化，如有必要，可以进行粗略校准。这些研究证明了造影剂技术的可行性，该技术可能对数十微米分辨率的代谢功能性核磁共振（fMRI）研究有用。 Magn Reson Med 49:609–614, 2003。© 2003 Wiley-Liss, Inc.
In this work we show that exogenous molecular hemoglobin (Hb) is an effective indicator of relative local oxygen tension in magnetic resonance (MR) microscopy studies in vivo. This approach is more sensitive than other MRI oximetry methods; it can be used at higher resolutions and in specimens with no blood oxygen level‐dependent (BOLD) effects. Using injection studies in flies, we show that Hb can permeate through relatively dense neural tissue, and that it is not obviously disruptive to physiology. Hb‐injected flies show large changes in signal intensity (40–50%) when external O2levels are manipulated artificially from 0% to 21%. Oxygen‐dependent contrast changes produced by exogenous Hb are detected inT2‐weighted imaging experiments, and can be roughly calibrated if necessary. These studies demonstrate the feasibility of a contrast agent technique that may be useful for functional MRI (fMRI) studies of metabolism at tens of microns resolution. Magn Reson Med 49:609–614, 2003. © 2003 Wiley‐Liss, Inc.