Controlling stem cell behavior with decellularized extracellular matrix scaffolds.
Controlling stem cell behavior with decellularized extracellular matrix scaffolds.
复制标题
用脱细胞外基质支架控制干细胞行为。
DOI：
10.1016/j.cossms.2016.02.001
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发表时间：
2016-08
影响因子：
11
通讯作者：
Christman KL
中科院分区：
文献类型：
--
作者：
Agmon G;Christman KL
脱细胞组织已成为一种常见的再生医学平台，多种材料正在学术实验室中进行研究，在动物研究中进行测试，并在临床上使用。理想情况下，当组织被脱细胞化时，天然细胞龛保持有许多与该特定组织的细胞自然相互作用的结构和生化线索。这使得脱细胞组织材料成为一个极好的平台，用于为细胞提供启动和维持分化为组织特异性谱系所需的信号。脱细胞化过程后剩余的细胞外基质（ECM）含有不可能合成产生的组织特异性微环境的组分。每个组织的ECM具有不同的组成和结构，因此具有独特的特性和影响细胞行为的潜力。本文综述了脱细胞组织材料的常用制备方法以及不同组织来源的脱细胞材料对细胞表型的影响。
Decellularized tissues have become a common regenerative medicine platform with multiple materials being researched in academic laboratories, tested in animal studies, and used clinically. Ideally, when a tissue is decellularized the native cell niche is maintained with many of the structural and biochemical cues that naturally interact with the cells of that particular tissue. This makes decellularized tissue materials an excellent platform for providing cells with the signals needed to initiate and maintain differentiation into tissue-specific lineages. The extracellular matrix (ECM) that remains after the decellularization process contains the components of a tissue specific microenvironment that is not possible to create synthetically. The ECM of each tissue has a different composition and structure and therefore has unique properties and potential for affecting cell behavior. This review describes the common methods for preparing decellularized tissue materials and the effects that decellularized materials from different tissues have on cell phenotype.