Subzero organ preservation: the dawn of a new ice age?
Subzero organ preservation: the dawn of a new ice age?
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DOI：
10.1097/mot.0000000000000403
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发表时间：
2017-06
影响因子：
2.2
通讯作者：
Uygun K
中科院分区：
文献类型：
--
作者：
Bruinsma BG;Uygun K
在这里，我们回顾了零度以下的器官保存领域，重点介绍了肝脏过冷的最新进展。器官保存正在从十年前的冰冻保存标准迅速转变为提高器官可用性和保存时间的技术。器官的长期保存将给器官移植领域带来巨大的好处，包括更好的器官分配，供受者之间的匹配，以及减少保存损伤和随后改善供体器官的使用。冰的形成已被证明是一个重要的限制因素，新技术试图使用低温保护剂和高度受控的冷却方案来控制或防止结冰。在过去的几十年里，人们使用了各种技术，包括真正的器官冷冻、玻璃化冷冻和零度以下的非冷冻或过冷。对于大多数技术来说，在长期低温保存后成功移植仍然是难以捉摸的。然而，过冷最近产生了第一个有希望的结果，在−6℃下进行了长达4天的过冷保存，获得了存活。随着器官保存领域的重大发展，零度保存领域也重新引起了人们的兴趣。虽然要使零度以下的保存可行仍需克服许多障碍，但新技术开始显示出它们在实现长期保存方面的潜力。
Herein, we review the field of subzero organ preservation with a focus on recent developments in hepatic supercooling. Organ preservation is making a rapid shift from the decade-old standard of storage on ice towards techniques that improve organ availability as well as preservation time. Long-term organ preservation would have tremendous benefits to the organ transplantation field, including better organ allocation, donor-recipient matching, as well as reduced preservation injury and subsequent improvement of donor organ use. The formation of ice has proven an important limiting factor and novel techniques attempt to control or prevent freezing using cryoprotective agents, and highly-controlled cooling regimens. Various techniques have been employed over the previous decades including true organ freezing, vitrification and subzero non-freezing, or supercooling. For most techniques, successful transplantation following long-term subzero preservation has remained elusive. Supercooling however recently delivered the first promising results, yielding survival after up to 4 days of supercooled preservation at −6 °C. As the field of organ preservation undergoes significant development, the field of subzero preservation also receives renewed interest. While many obstacles remain to be overcome to make subzero preservation feasible, novel techniques are beginning to show their potential in achieving long-term preservation.