Antigen-Presenting Cells Are Stratified Within Normal Human Corneas and Are Rapidly Mobilized During Ex Vivo Viral Infection
Antigen-Presenting Cells Are Stratified Within Normal Human Corneas and Are Rapidly Mobilized During Ex Vivo Viral Infection
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DOI：
10.1167/iovs.13-13523
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发表时间：
2014-02-01
影响因子：
4.4
通讯作者：
Hendricks, Robert L.
中科院分区：
文献类型：
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作者：
Knickelbein, Jared E.;Buela, Kristine-Ann;Hendricks, Robert L.
目的.目的：明确正常人角膜组织中抗原呈递细胞（APC）的表型和定位，以及APC对单纯疱疹病毒1型（HSV 1）感染的反应。通过荧光共聚焦显微镜和流式细胞术分析供体人角膜组织，以确定组织驻留APC的表型和位置。共聚焦荧光显微镜观察角膜APC对HSV-1感染的反应。CD 11 c（+）树突状细胞（DCs）和CD 207（+）朗格汉斯细胞（LCs）主要分布于角膜基底上皮，CD 68（+）巨噬细胞主要分布于角膜前基质。大多数DC表达主要组织相容性复合物II类。角膜常驻APC与HSV-1感染的角膜细胞在离体感染后8 - 16小时内共定位。在人角膜中发现的APC的分层与先前在小鼠中报道的非常相似，证实了用于角膜APC研究的鼠模型的相关性。此外，角膜驻留APC能够快速移动到角膜内的创伤和HSV-1感染部位。
PURPOSE. To define the phenotype and location of antigen-presenting cells (APCs) in normal donor human corneas, and to assess the response of APC to herpes simplex virus type 1 (HSV1) infection.METHODS. Donor human corneal tissue was analyzed by fluorescence confocal microscopy and flow cytometry to determine the phenotype and location of tissue-resident APCs. Confocal fluorescence microscopy was also utilized to investigate the response of corneal resident APCs to ex vivo infection with HSV-1.RESULTS. CD11c(+) dendritic cells (DCs) and CD207(+) Langerhans cells (LCs) were situated predominantly in the basal epithelium and CD68(+) macrophages in the anterior stroma of human corneas. The majority of DCs expressed major histocompatibility complex class II. Corneal resident APCs colocalized with HSV-1-infected corneal cells within 8 to 16 hours of ex vivo infection.CONCLUSIONS. The stratification of APCs found in human corneas is very similar to that previously reported in mice, confirming the relevance of murine models for the study of corneal APCs. Furthermore, corneal resident APCs are capable of rapidly mobilizing to the site of trauma and HSV-1 infection within the cornea.