Differences in the interleukin‐2 (IL‐2) receptor system in human and mouse: α chain is required for formation of the functional mouse IL‐2 receptor
Differences in the interleukin‐2 (IL‐2) receptor system in human and mouse: α chain is required for formation of the functional mouse IL‐2 receptor
复制标题
人类和小鼠白介素-2 (IL-2) 受体系统的差异：α 链是形成功能性小鼠 IL-2 受体所必需的
DOI：
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发表时间：
1995
影响因子：
5.4
通讯作者：
K. Sugamura
中科院分区：
文献类型：
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作者：
Tadayoshi Nemoto;T. Takeshita;N. Ishii;M. Kondo;M. Higuchi;Susumu Satomi;M. Nakamura;S. Mori;K. Sugamura
与小鼠白介素2受体亚基的重组表明，小鼠白介素2受体复合体与人复合体在α链上对功能性小鼠受体复合体的需求不同。小鼠外源性α和β链的异三聚体复合体和小鼠淋巴系BW5147细胞上的内源性γ链显示出与IL-2高亲和力结合IL-2的能力，导致参与IL-2依赖信号的胞浆酪氨酸激酶JAK3的酪氨酸磷酸化。然而，外源性将β链与内源性γ链一起导入BW5147细胞，既不能与IL-2结合，也不能诱导IL-2诱导的信号转导，这与人βγ异源二聚体不同。小鼠脾CD8+细胞最初没有α链，只有当α链被诱导表达时，才显示出IL-2依赖的细胞增殖。综上所述，这些结果表明，具有功能的小鼠IL-2受体复合体必然包括α链，并且免疫反应过程中CD8+T细胞生长的调节依赖于α链的表达。
Reconstitution with mouse interleukin‐2 (IL‐2) receptor subunits demonstrated that the mouse IL‐2 receptor complex was different from the human complex in the α chain requirement for the functional mouse receptor complex. The heterotrimeric complex of the mouse exogenous α and β chains and the endogenous γ chain on mouse lymphoid BW5147 cells showed the ability to bind IL‐2 with high affinity, resulting in IL‐2‐induced tyrosine phosphorylation of a cytosolic tyrosine kinase, JAK3, which is involved in IL‐2‐dependent signals. Exogenous introduction of the β chain with the endogenous γ chain, however, could neither confer appreciable IL‐2 binding nor IL‐2‐induced signal transduction on BW5147 cells, unlike the human βγ heterodimer. Mouse spleen CD8+ cells, not having the α chain initially, showed IL‐2‐dependent cell proliferation only when expression of the α chain was induced. Collectively, these results illustrate that the functional mouse IL‐2 receptor complex necessarily includes the α chain, and that the regulation of CD8+ T cell growth during immune reaction depends upon α chain expression.