Human herpesvirus-6A gQ1 and gQ2 are critical for human CD46 usage
Human herpesvirus-6A gQ1 and gQ2 are critical for human CD46 usage
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DOI：
10.1111/1348-0421.12110
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发表时间：
2014-01-01
影响因子：
2.6
通讯作者：
Mori, Yasuko
中科院分区：
文献类型：
--
作者：
Jasirwan, Chyntia;Furusawa, Yoshikazu;Mori, Yasuko
基于遗传和抗原差异以及它们的细胞嗜性，人疱疹病毒-6（HHV-6）已被分为两个变体，HHV-6A和HHV-6 B。最近，这些变种被重新归类为两个不同的物种。HHV-6A糖蛋白复合物gH/gL/gQ 1/gQ 2与其细胞受体CD 46结合;然而，HHV-6 B中相应的复合物很少与CD 46结合。为了确定糖蛋白复合物中哪些病毒分子决定HHV-6A-CD 46结合，HHV-6A复合物的每个分子（即，gH、gL、gQ 1或gQ 2）被相应的HHV-6 B分子取代，并检查被取代的蛋白质掺入复合物的能力和复合物结合CD 46的能力。发现当所有四种糖蛋白表达时，它们能够形成四聚体复合物。HHV-6A gH/gL/gQ 1/gQ 2复合物被HHV-6 B gQ 1或gQ 2分子取代后形成的复合物几乎不与CD 46结合，而HHV-6A gH或gL被HHV-6 B分子取代后形成的复合物则与CD 46结合，表明HHV-6A gQ 1和gQ 2在CD 46结合中起重要作用。
Based on genetic and antigenic differences and on their cell tropism, human herpes virus-6 (HHV-6) has been classified into two variants, HHV-6A and HHV-6B. Recently, these variants were re-classified as two different species. The HHV-6A glycoprotein complex, gH/gL/gQ1/gQ2 binds to its cellular receptor, CD46; however, the corresponding complex in HHV-6B rarely binds to CD46. To determine which viral molecules in the glycoprotein complex determine HHV-6A-CD46 binding, each molecule of the HHV-6A complex (i.e., gH, gL, gQ1, or gQ2) was replaced with the corresponding HHV-6B molecule, and the ability of the replaced protein to be incorporated into the complex and the ability of the complex to bind CD46 were examined. It was found that when all four glycoproteins were expressed, they were able to form a tetrameric complex. However, a complex formed by HHV-6A gH/gL/gQ1/gQ2 complexes replaced with HHV-6B gQ1 or gQ2 scarcely bind CD46, whereas HHV-6A complexes in which gH or gL was replaced with the HHV-6B molecules did bind it. These results indicate that HHV-6A gQ1 and gQ2 play an important role in CD46 binding.