Memory T-lymphocyte survival does not require T-cell receptor expression
Memory T-lymphocyte survival does not require T-cell receptor expression
复制标题
DOI：
10.1073/pnas.0806289106
复制
发表时间：
2008-12-23
影响因子：
11.1
通讯作者：
Labrecque, Nathalie
中科院分区：
文献类型：
--
作者：
Leignadier, Julie;Hardy, Marie-Pierre;Labrecque, Nathalie
控制记忆T（Tm）细胞寿命的因素仍然定义不清，它们的识别对于设计提供长期抗感染保护的疫苗至关重要。Tm细胞具有在不存在T细胞受体（TCR）-MHC相互作用的情况下存活的能力。这并不排除TCR内在配体非依赖性组成性信号传导在Tm细胞稳态中的可能作用。使用一种独特的TCR四环素诱导表达系统，我们表明，TCR表达的消融，消除任何可能的信号通过TCR，不影响抗原特异性的CD 8（+）Tm细胞的生存和自我更新，即使它们必须与内源性T细胞竞争生存因子。此外，即使在没有TCR-MHC相互作用的情况下长期维持，CD 8（+）Tm细胞功能也没有改变。此外，我们的研究结果表明，一个亚群的CD 4（+）Tm细胞可以在非淋巴细胞减少宿主的TCR表达的情况下存活。
The factors controlling memory T (Tm)-cell longevity are still poorly defined, and their identification is pivotal to the design of a vaccine conferring long-term protection against infection. Tm cells have the ability to survive in the absence of the T-cell receptor (TCR)-MHC interaction. This does not exclude a possible role for TCR-intrinsic ligand-independent constitutive signaling in Tm-cell homeostasis. Using a unique TCR tetracycline-inducible expression system, we show that the ablation of TCR expression, which abrogates any possible signaling via the TCR, did not influence the survival and self-renewal of antigen-specific CD8(+) Tm cells even when they have to compete with endogenous T cells for survival factors. Moreover, CD8(+) Tm-cell functionality was not altered even on prolonged maintenance in the absence of TCR-MHC interactions. Furthermore, our results show that a subset of CD4(+) Tm cells can survive in the absence of TCR expression in nonlymphopenic hosts.