Determination of Niacinamide in Lotions and Creams Using Liquid-Liquid Extraction and High-Performance Liquid Chromatography
Determination of Niacinamide in Lotions and Creams Using Liquid-Liquid Extraction and High-Performance Liquid Chromatography
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采用液液萃取和高效液相色谱法测定乳液和霜剂中的烟酰胺
DOI：
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
H. Rossi
中科院分区：
文献类型：
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作者：
Karyn M. Usher;C. R. Simmons;Daniel W. Keating;H. Rossi
化学分离是本科化学课程的重要组成部分。大二学生经常在有机化学课上接触到液液萃取，但在荣誉普通化学或定量分析课上，液液萃取作为定量样品制备方法很少被介绍。本实验提供了一种相关的、有趣的方法，使用液-液萃取和高效液相色谱（HPLC）分析流行的乳液和面霜的烟酰胺含量。完成实验后，学生将对提取技术、比尔-朗伯定律和高效液相色谱有更好的了解。对玉兰油原液的加标回收率为99.22%，标准偏差为0.01%。
Chemical separations are an important part of an undergraduate chemistry curriculum. Sophomore students often get experience with liquid–liquid extraction in organic chemistry classes, but liquid–liquid extraction is not as often introduced as a quantitative sample preparation method in honors general chemistry or quantitative analysis classes. This experiment offers a relevant, interesting way to use liquid–liquid extraction and high-performance liquid chromatography (HPLC) to analyze popular lotions and creams for niacinamide content. After completing the experiment, students will have a better understanding of the extraction technique, Beer–Lambert law, and HPLC. The percent recovery from a spiked sample of Olay Original Active Hydrating Beauty Fluid was found to be 99.22% with a standard deviation of 0.01%.