Rat Astrocytes are More Supportive for Mouse OPC Self-Renewal than Mouse Astrocytes in Culture
Rat Astrocytes are More Supportive for Mouse OPC Self-Renewal than Mouse Astrocytes in Culture
复制标题
培养中的大鼠星形胶质细胞比小鼠星形胶质细胞更支持小鼠 OPC 自我更新
DOI：
10.1002/dneu.22476
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发表时间：
2017-09-01
影响因子：
3
通讯作者：
Yang, Junlin
中科院分区：
文献类型：
--
作者：
Cheng, Xuejun;Xie, Binghua;Yang, Junlin
小鼠原代少突胶质前体细胞（OPCs）越来越多地被用于研究转基因或突变小鼠模型中观察到的少突胶质细胞分化和轴突髓鞘形成表型变化的分子机制。然而，与大鼠相比，用简单的脑组织分离培养方法分离小鼠的OPCs要困难得多。迄今为止，OPC制备中物种差异的机制仍然不清楚。在本研究中，我们发现大鼠的星形胶质细胞比小鼠的星形胶质细胞在体外促进OPC增殖和存活的作用更强。小鼠星形胶质细胞在培养中表现出明显较弱的活力，维持OPC自我更新的潜力降低，这一点在用大鼠或小鼠星形胶质细胞的条件培养基培养OPC时得到了证实。这些结果解释了为什么在小鼠中枢神经系统组织中难以实现OPCs和星形胶质细胞的分层培养。基于上述发现，我们采用灭活的大鼠星形胶质细胞作为饲养细胞，支持小鼠皮质OPCs的自我更新，制备高纯度小鼠OPCs。(C) 2016 Wiley期刊公司
Mouse primary oligodendrocyte precursor cells (OPCs) are increasingly used to study the molecular mechanisms underlying the phenotype changes in oligodendrocyte differentiation and axonal myelination observed in transgenic or mutant mouse models. However, mouse OPCs are much more difficult to be isolated by the simple dissociation culture of brain tissues than their rat counterparts. To date, the mechanisms underlying the species difference in OPC preparation remain obscure. In this study, we showed that astrocytes from rats have a stronger effect than those from mouse in promoting OPC proliferation and survival in vitro. Mouse astrocytes displayed significantly weaker viability in culture and reduced potential in maintaining OPC self-renewal, as confirmed by culturing OPCs with conditioned media from rat or mouse astrocytes. These results explained the reason for why stratified cultures of OPCs and astrocytes are difficult to be achieved in mouse CNS tissues. Based on these findings, we adopted inactivated rat astrocytes as feeder cells to support the self-renewal of mouse cortical OPCs and preparation of high-purity mouse OPCs. (C) 2016 Wiley Periodicals, Inc.