Quantitative optical imaging of vascular response in vivo in a model of peripheral arterial disease.
Quantitative optical imaging of vascular response in vivo in a model of peripheral arterial disease.
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DOI：
10.1152/ajpheart.00362.2013
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发表时间：
2013-10
期刊：
影响因子：
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通讯作者：
K. M. Poole;J. Tucker-Schwartz;W. Sit;Alex J. Walsh;C. Duvall;M. Skala
中科院分区：
文献类型：
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作者：
K. M. Poole;J. Tucker-Schwartz;W. Sit;Alex J. Walsh;C. Duvall;M. Skala
小鼠后肢缺血 (HLI) 模型已广泛用于研究侧支血管形成和测试外周动脉疾病的治疗方法，但缺乏活体分析血管结构和功能的定量技术。为了满足这一需求，开发了非侵入性定量光学成像技术来评估小鼠 HLI 模型的恢复时间过程。在诱导 HLI 后，使用高光谱成像和光学相干断层扫描 (OCT) 对麻醉小鼠的血红蛋白氧饱和度和微血管形态以及血流进行无创成像。高光谱成像早在股动脉结扎后 3 天就检测到缺血爪中的血红蛋白饱和度显着增加（P < 0.01），并且在术后 14 天通过 OCT 首次检测到远端血流量显着增加（P < 0.01）。内收肌脉管系统的活体 OCT 图像显示出螺旋形侧支血管，这是对 HLI 的动脉生成反应的特征。高光谱成像和 OCT 数据彼此显着相关，并且与激光多普勒灌注成像 (LDPI) 和组织氧合传感器数据显着相关 (P < 0.01)。然而，OCT 测量获取了深度分辨信息，并揭示了手术后更持续的血流缺陷，而这些缺陷可能被更表面的测量（LDPI、高光谱成像）所掩盖。因此，活体 OCT 可能为缺血性肢体恢复的后期提供强有力的生物标志物。这项工作验证了通过高光谱成像和 OCT 对功能和形态数据的非侵入性采集。这些技术共同为心血管研究人员提供了关于活体小鼠 HLI 恢复时间动态的前所未有的全面视图。
The mouse hind limb ischemia (HLI) model is well established for studying collateral vessel formation and testing therapies for peripheral arterial disease, but there is a lack of quantitative techniques for intravitally analyzing blood vessel structure and function. To address this need, non-invasive, quantitative optical imaging techniques were developed to assess the time-course of recovery in the mouse HLI model. Hyperspectral imaging and optical coherence tomography (OCT) were used to non-invasively image hemoglobin oxygen saturation and microvessel morphology plus blood flow, respectively, in the anesthetized mouse after induction of HLI. Hyperspectral imaging detected significant increases in hemoglobin saturation in the ischemic paw as early as 3 days after femoral artery ligation (P < 0.01), and significant increases in distal blood flow were first detected with OCT 14 days postsurgery (P < 0.01). Intravital OCT images of the adductor muscle vasculature revealed corkscrew collateral vessels characteristic of the arteriogenic response to HLI. The hyperspectral imaging and OCT data significantly correlated with each other and with laser Doppler perfusion imaging (LDPI) and tissue oxygenation sensor data (P < 0.01). However, OCT measurements acquired depth-resolved information and revealed more sustained flow deficits following surgery that may be masked by more superficial measurements (LDPI, hyperspectral imaging). Therefore, intravital OCT may provide a robust biomarker for the late stages of ischemic limb recovery. This work validates non-invasive acquisition of both functional and morphological data with hyperspectral imaging and OCT. Together, these techniques provide cardiovascular researchers an unprecedented and comprehensive view of the temporal dynamics of HLI recovery in living mice.