Three-dimensional image analysis of plugging at the septal pore by Woronin body during hypotonic shock inducing hyphal tip bursting in the filamentous fungus Aspergillus oryzae
Three-dimensional image analysis of plugging at the septal pore by Woronin body during hypotonic shock inducing hyphal tip bursting in the filamentous fungus Aspergillus oryzae
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DOI：
10.1016/j.bbrc.2005.03.233
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发表时间：
2005-06-17
影响因子：
3.1
通讯作者：
Kitamoto, K
中科院分区：
文献类型：
--
作者：
Maruyama, JI;Juvvadi, PR;Kitamoto, K
我们观察到，在琼脂培养基上生长的丝状真菌米曲霉（Aspergillus oryzae）在被水淹没后很快就从菌丝尖端爆发出细胞质成分。Woronin小体是一种特殊的细胞器，它堵塞与裂解室相邻的间隔孔，以防止细胞质的广泛损失。与编码Woronin小体主要蛋白的神经孢子虫HEX1基因同源的a . oryzae Aohex1基因与DsRed2融合表达，实现Woronin小体的可视化。共聚焦显微镜和三维重建图像显示，鼻中隔孔是一个被egfp融合分泌蛋白RNase T1的绿色荧光包围的黑暗区域。双荧光标记显示在低渗休克时，沃洛宁体堵塞了靠近破裂的根尖室的间隔孔。Aohex1基因的破坏导致Woronin小体的消失和缺陷，以防止低渗休克时细胞质的广泛损失。(c) 2005爱思唯尔公司版权所有。
We observed that the filamentous fungus, Aspergillus oryzae, grown on agar media burst out cytoplasmic constituents from the hyphal tip soon after flooding with water. Woronin body is a specialized organelle known to plug the septal pore adjacent to the lysed compartment to prevent extensive loss of cytoplasm. A. oryzae Aohex1 gene homologous to Neurospora crassa HEX1 gene encoding a major protein in Woronin body was expressed as a fusion with DsRed2, resulting in visualization of Woronin body. Confocal microscopy and three-dimensional reconstruction of images visualized the septal pore as a dark region surrounded by green fluorescence of EGFP-fused secretory protein, RNase T1, on the septum. Dual fluorescent labeling revealed the plugging of the septal pores adjacent to the lysed apical compartments by Woronin bodies during hypotonic shock. Disruption of Aohex1 gene caused disappearance of Woronin bodies and the defect to prevent extensive loss of cytoplasm during hypotonic shock. (c) 2005 Elsevier Inc. All rights reserved.