Expression of a conifer COBRA-like gene ClCOBL1 from Chinese fir (Cunninghamia lanceolata) alters the leaf architecture in tobacco
Expression of a conifer COBRA-like gene ClCOBL1 from Chinese fir (Cunninghamia lanceolata) alters the leaf architecture in tobacco
复制标题
杉木 (Cunninghamia lanceolata) 中针叶树 COBRA 样基因 ClCOBL1 的表达改变了烟草的叶片结构
DOI：
10.1016/j.plaphy.2013.06.013
复制
发表时间：
2013-09-01
影响因子：
6.5
通讯作者：
Wang, Guifeng
中科院分区：
文献类型：
--
作者：
Gao, Yan;Bian, Liming;Wang, Guifeng
细胞壁在植物细胞的形态、对生物和非生物胁迫的防御反应以及器官的机械特性等方面起着至关重要的作用。COBRA基因编码一种糖基磷脂酰肌醇（GPI）锚定蛋白，具有调节植物细胞中纤维素沉积和定向细胞扩增的能力。本文报道了杉木（Cunninghamia lanceolata（Lamb.）Hook）。ClCOBL 1属于COBRA蛋白家族的木本植物特异性分支，具有几个保守的基序。表达谱分析表明ClCOBL 1在形成层区域高水平表达，但在形成层区域高水平表达。ClCOBL 1蛋白主要定位于细胞壁和质膜。在烟草植株中过表达ClCOBL 1产生改变的叶片近轴-远轴图案和短的，肿胀的花冠管。ClCOBL 1过表达基因改变的叶片结构与叶片近轴-远轴同一性基因的差异表达有关。这些结果表明，ClCOBL 1可能通过影响近轴和远轴同源基因的表达而参与决定叶片的背腹性和各向异性扩展。(c)2013年Elsevier Masson SAS。All rights reserved.
The cell wall plays crucial roles in establishing the morphology of the plant cell, defence response to biotic and abiotic stresses, and mechanical properties of organs. The COBRA gene encodes a putative glycosylphosphatidylinositol (GPI)-anchored protein that possesses the ability to modulate cellulose deposition and orient cell expansion in plant cell. We reported here the functional characterization of ClCOBL1, a conifer COBRA-like gene from the differentiating xylem of Chinese fir (Cunninghamia lanceolata (Lamb.) Hook). ClCOBL1 belonged to a woody plant-specific clade of the COBRA protein family with several conserved motifs. Expression pattern demonstrated that ClCOBL1 was constitutively expressed but with high level in cambium region. ClCOBL1 protein was mainly located in the cell wall and plasma membrane. Overexpression of ClCOBL1 in tobacco plants yielded altered leaf adaxial-abaxial patterning and short, swollen corolla tubes. The changed leaf architecture in the ClCOBL1 overexpressors was associated with the differential expression of leaf adaxial-abaxial identity genes. Our results indicated that ClCOBL1 was involved in the determination of leaf dorsoventrality and anisotropic expansion possibly by affecting the expression of adaxial and abaxial identity genes. (c) 2013 Elsevier Masson SAS. All rights reserved.