Infectious cDNA clones of tick-borne encephalitis virus European subtype prototypic strain Neudoerfl and high virulence strain Hypr
Infectious cDNA clones of tick-borne encephalitis virus European subtype prototypic strain Neudoerfl and high virulence strain Hypr
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DOI：
10.1099/0022-1317-78-5-1049
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发表时间：
1997-05-01
影响因子：
3.8
通讯作者：
Heinz, FX
中科院分区：
文献类型：
--
作者：
Mandl, CW;Ecker, M;Heinz, FX
构建了两株TBE病毒的感染性cDNA克隆，即欧洲亚型原型株Neudoerfl和与之密切相关但毒力更强的Hypr株。重组构建体由在T7启动子元件控制下稳定插入细菌质粒pBR 322中的cDNA组成。成功地克隆了TBE病毒Neudoerfl株的基因组，包括全长cDNA和两个部分cDNA。在菌株Hypr的情况下，基因组由对应于基因组的5 ′-和3 ′-末端一半的两个cDNA克隆表示。高度感染性的RNA可以从全长cDNA克隆或从体外连接的部分克隆产生，以在T7转录之前形成全长cDNA模板。重组子代病毒的生物学特性，包括毒力特征，与相应的亲本病毒株没有区别。因此，所描述的感染性cDNA克隆代表了用于TBE病毒特异性诱变的有用且可靠的实验系统。
Infectious cDNA clones of two strains of tick-borne encephalitis (TBE) virus, i.e. European subtype prototypic strain Neudoerfl and the closely related but more virulent strain Hypr, were constructed. The recombinant constructs consisted of cDNAs stably inserted into the bacterial plasmid pBR322 under the control of T7 promoter elements. The genome of TBE virus strain Neudoerfl was successfully cloned, both as a full-length cDNA and as two partial cDNAs. In the case of strain Hypr, the genome is represented by two cDNA clones corresponding to the 5'- and 3'-terminal halves of the genome. Highly infectious RNAs can be produced from the full-length cDNA clone or from the partial clones ligated in vitro to form full-length cDNA templates prior to T7 transcription. The biological properties of the recombinant progeny viruses, including virulence characteristics, were indistinguishable from the corresponding parent virus strains. Thus, the described infectious cDNA clones represent a useful and reliable experimental system for the specific mutagenesis of TBE virus.