Decreased frequency of HCV core-specific peripheral blood mononuclear cells with type 1 cytokine secretion in chronic hepatitis C
Decreased frequency of HCV core-specific peripheral blood mononuclear cells with type 1 cytokine secretion in chronic hepatitis C
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DOI：
10.1016/s0168-8278(99)80307-9
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发表时间：
1999-12-01
影响因子：
25.7
通讯作者：
Spengler, U
中科院分区：
文献类型：
--
作者：
Lechmann, M;Woitas, RP;Spengler, U
背景/目的：由于丙型肝炎感染的结果似乎与对 HCV 核心蛋白的免疫反应相关，因此本研究的目的是研究 T 细胞对丙型肝炎病毒核心和核心衍生抗原的反应。方法：由于这种反应可能受到细胞因子分泌差异的重要调节，我们确定了响应重组 HCV 前蛋白而分泌 IL-2、IL-4、IL-10 和 IFN-gamma 的外周血单核细胞 (PBMC) 的数量和一组 19 个核心衍生肽，使用 ELI-Spot 技术，对两组患者进行了研究： A 组：11 名既往患有自限性 HCV 感染的患者； B 组：12 名患有慢性丙型肝炎的患者。结果：在 B 组中，IFN-γ 显着减少，在用核心蛋白刺激后（0.0830+/-0.083 SFC 对比 1.3+/-0.4 SFC/10(5) PBMC；p=0.005）和用核心衍生肽刺激后（1.3+/-0.5 对比 4.4+/-1.1 SFC），均可以检测到斑点形成细胞 (SFC) SFC/10(-5)PBMC；p=0.007)。通过分析对每个单一肽的细胞因子反应，我们发现 A 组中对肽 aa 39-63 和 aa 148-172 有 IFN-gamma 反应，但 B 组中没有 (p
Background/Aim: Since the outcome of hepatitis C infection appears to be correlated with the immune response to the HCV core protein, the aim of this study was to investigate the T cell response to hepatitis C virus core and core-derived antigens.Methods: As this response may be regulated importantly by differential secretion of cytokines, we determined the number of peripheral blood mononuclear cells (PBMC) that secreted IL-2, IL-4, IL-10, and IFN-gamma in response to a recombinant HCV fore protein and a panel of 19 core-derived peptides, using the ELI-Spot-technique, Two groups of patients were studied: group A: 11 patients with previously self-limited HCV infection; group B: 12 patients with chronic hepatitis C.Results: In group B significantly less IFN-gamma, spot forming cells (SFC) could be detected, both after stimulation with the core protein (0.0830+/-0.083 SFC vs, 1.3+/-0.4 SFC/10(5) PBMC; p=0.005) and with the core-derived peptides (1.3+/-0.5 vs. 4.4+/-1.1 SFC SFC/10(-5) PBMC; p=0.007). By analyzing the cytokine response to each single peptide, we found IFN-gamma responses to peptides aa 39-63 and aa 148-172 in group A but not in group B (p