Oxidative stress in cancer-bearing dogs assessed by measuring serum malondialdehyde
Oxidative stress in cancer-bearing dogs assessed by measuring serum malondialdehyde
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DOI：
10.1186/1746-6148-9-101
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发表时间：
2013-05-11
影响因子：
2.6
通讯作者：
Boonsiri, Patcharee
中科院分区：
文献类型：
--
作者：
Macotpet, Arayaporn;Suksawat, Fanan;Boonsiri, Patcharee
背景：氧化应激，活性氧（ROS）的过量，引起脂质过氧化，导致细胞和组织损伤。它可能与狗的癌症的发展和进展有关。丙二醛（MDA）是脂质过氧化的最终产物，通常被用作氧化应激的标志物。本研究的目的是通过测量血清丙二醛水平来评估患癌犬的氧化应激。在主人同意的情况下，所有客户拥有的狗都在孔敬大学兽医学院兽医教学医院接受了体检，以确定其健康状况。取患癌犬（N = 80）和临床正常犬（N = 101）血样，测定MDA水平。此外，测定全血细胞计数、肌酐和丙氨酸转氨酶。结果：患癌犬血清MDA明显高于临床正常犬（平均+/- SD分别为4.68 +/- 1.32 mu mol/L和2.95 +/- 0.61 mu mol/L, p < 0.001）。癌犬的细胞体积（平均+/- SD， 36.18 +/- 7.65% vs 44.84 +/- 5.54%）、血红蛋白（平均+/- SD， 11.93 +/- 2.88 g% vs 15.17 +/- 2.00 g%）和红细胞(中位数（IQA）， 6.05 （2.15) vs 8.09(1.34)）均显著低于临床正常犬。然而，白细胞的情况正好相反(IQA中位数为18.20 （11.95)vs 14.90(5.10)）。各组间肌酐和丙氨酸转氨酶水平均无显著差异。结论：本研究支持氧化应激与犬类多种癌症相关的结论，因为患癌犬的血清丙二醛水平明显高于临床正常犬。
Background: Oxidative stress, an excess of reactive oxygen species (ROS), causes lipid peroxidation resulting in cell and tissue damages. It may be associated with the development and progression of cancers in dogs. Malondialdehyde (MDA), the end product of lipid peroxidation, is commonly used as a marker of oxidative stress. The objective of this study was to assess oxidative stress in cancer-bearing dogs by measuring serum MDA levels. All client-owned dogs underwent physical examination at the Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Khon Kaen University to determine the health status with the owner's consent. Blood samples of cancer-bearing dogs (N = 80) and clinically normal dogs (N = 101) were obtained and subjected for determination of MDA levels. In addition, complete blood count, creatinine, and alanine aminotransferase were measured.Results: Serum MDA was significantly higher in cancer-bearing dogs than in clinically normal dogs (mean +/- SD, 4.68 +/- 1.32 mu mol/L vs 2.95 +/- 0.61 mu mol/L, respectively; p < 0.001). Packed cell volume (mean +/- SD, 36.18 +/- 7.65% vs 44.84 +/- 5.54%), hemoglobin (mean +/- SD, 11.93 +/- 2.88 g% vs 15.17 +/- 2.00 g%) and red blood cells (median (IQA), 6.05 (2.15) vs 8.09 (1.34)) were all significantly lower in cancer-bearing dogs than in clinically normal dogs. However, the reverse was true for white blood cells (median (IQA), 18.20 (11.95) vs 14.90 (5.10)). Neither creatinine nor alanine aminotransferase levels were significantly different between groups.Conclusions: This study supports the conclusion that oxidative stress is associated with many types of cancers in dogs, as serum MDA levels were significantly higher in cancer-bearing dogs compared to clinically normal dogs.