Central nervous system chemokine expression during Theiler's virus-induced demyelinating disease
Central nervous system chemokine expression during Theiler's virus-induced demyelinating disease
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DOI：
10.3109/13550289909021292
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发表时间：
1999-12-01
影响因子：
3.2
通讯作者：
Karpus, WJ
中科院分区：
文献类型：
--
作者：
Hoffman, LM;Fife, BT;Karpus, WJ
Theiler氏鼠脑脊髓炎病毒是一种地方性鼠病原体，在易感小鼠品系中诱导中枢神经系统脱髓鞘疾病。该疾病的特征是中枢神经系统单核细胞浸润，并表现为慢性进行性瘫痪。用ELISA和RT-PCR方法检测了感染Theiler小鼠脑脊髓炎病毒的小鼠中枢神经系统中CC和C-x-C趋化因子的表达。MCP-1和MIP-1 α蛋白的中枢神经系统表达在SJL小鼠的Theiler鼠脑脊髓炎病毒感染后第11天是明显的，并且在整个疾病进展中持续。MIP-1 α、RANTES、MCP-1、C10、IP-10和MIP-1 β mRNA在Theiler小鼠脑脊髓炎病毒感染后的中枢神经系统中特异性表达，而非外周。这与临床疾病的发展有关。这些数据表明，多种趋化因子在病毒感染后的特定时间的表达与脱髓鞘疾病有关。
Theiler's murine encephalomyelitis virus is an endemic murine pathogen that induces a demyelinating disease of the central nervous system in susceptible mouse strains. The disease is characterized by central nervous system mononuclear cell infiltration and presents as chronic, progressive paralysis. The expression of CC and C-x-C chemokines in the central nervous system of Theiler's murine encephalomyelitis virus-infected mice was examined throughout the disease course by ELISA and RT-PCR analysis. Central nervous system expression of MCP-1 and MIP-1 alpha protein was evident by day 11 post Theiler's murine encephalomyelitis virus infection of SJL mice and continued throughout disease progression. MIP-1 alpha, RANTES, MCP-1, C10, IP-10, and MIP-1 beta mRNA was specifically expressed in the central nervous system and not the periphery following Theiler's murine encephalomyelitis virus infection. This was associated with development of clinical disease. These data suggest that the expression of multiple chemokines at particular times following viral infection is associated with demyelinating disease.