Strontium-containing apatite/polylactide composites enhance bone formation in osteopenic rabbits
Strontium-containing apatite/polylactide composites enhance bone formation in osteopenic rabbits
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DOI：
10.1016/j.actbio.2015.07.044
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发表时间：
2015-10-15
影响因子：
9.7
通讯作者：
Bruijn, Joost D.
中科院分区：
文献类型：
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作者：
Luo, Xiaoman;Barbieri, Davide;Bruijn, Joost D.
锶（Sr）已被证明有利于骨形成，临床上用于治疗骨质疏松症。我们以前曾报道过，在磷灰石/聚乳酸复合材料中加入锶可以增强BMP诱导的异位部位植入物周围骨形成。在这项研究中，我们的目的是调查的有效性锶添加对局部骨形成的骨质疏松症。将不同Sr含量的磷灰石/聚乳酸复合颗粒负载等量rhBMP-2，分别植入健康家兔（Con）和双侧卵巢切除并每日注射糖皮质激素（OP）的家兔体内，共植入12周。组织学和组织形态计量学研究了Sr对两组BMP诱导骨最终体积的潜在影响。当植入物不含Sr时，OP植入物中形成的新生骨显著少于Con组，表明OP动物中BMP诱导的成骨受损。在磷灰石中低至0.5摩尔%的Sr取代使OP植入物的骨体积增加至与Con组相当的水平，表明Sr添加对OP动物局部骨形成具有积极作用。此外，在OP植入物中，更多的脂肪组织形成与软骨组织的出现平行，这表明OP动物中干细胞的分化潜力可能已经转向脂肪生成和软骨生成。从这些结果中，我们得出结论，除了使用锶，以提高周围的种植体骨质疏松症值得进一步study.Statement of SignificanceThe受损的骨愈合能力的骨质疏松患者可能会导致不良的骨整合和手术失败的情况下，种植体被放置。在这项研究中，我们的目的是加强骨形成种植体周围在这种情况下，通过添加锶作为刺激。与其他研究不同的是，样品负载rhBMP-2并植入异位部位（新西兰兔的脊髓肌肉），以排除传导性骨修复的影响。结果表明，锶的加入可以促进BMP-2诱导的骨形成在骨质疏松兔的种植体上的水平与健康兔相当。其次，我们在骨质减少的植入物中观察到更多的脂肪组织和软骨组织，这表明脂肪生成和软骨生成在骨质减少/骨质疏松症中的作用。(C)2015由Elsevier Ltd.代表Acta Materialia Inc.出版。
Strontium (Sr) has been shown to favor bone formation and is used clinically to treat osteoporosis. We have previously reported that Sr addition in apatite/polylactide composites could enhance the BMP-induced bone formation around implants at ectopic site in healthy animals. In this study we aimed to investigate the effectiveness of Sr addition on the local bone formation in osteoporosis. Apatite/polylactide composite granules with different Sr content were loaded with equal amount of rhBMP-2 and implanted intramuscularly in healthy rabbits (Con) and rabbits that received bilateral ovariectomy and daily injection of glucocorticoid (OP) for 12 weeks. The potential effect of Sr on the final volume of BMP-induced bone in both groups was investigated histologically and histomorphometrically. The de novo bone formed in OP implants was significantly less than in Con group when the implants contained no Sr, indicating that the BMP-induced osteogenesis was impaired in OP animals. Sr substitution as low as 0.5 mol% in apatite increased the bone volume in OP implants to levels comparable to that in the Con group, indicating a positive effect of Sr addition on the local bone formation in OP animals. In addition, more adipose tissue formed in parallel with the appearance of cartilage tissue in OP implants, suggesting that the differentiation potential of stem cell in OP animals may have shifted towards adipogenesis and chondrogenesis. From these results, we conclude that the use of Sr addition to enhance the bone growth surrounding implants in osteoporosis merits further study.Statement of SignificanceThe impaired bone healing capacity of osteoporotic patients might result in poor osteointegration and surgical failure in case implants are placed. In this study we aimed to enhance the bone formation around implants under such scenario by adding strontium as the stimulus. Different from other studies, the samples were loaded with rhBMP-2 and implanted at an ectopic site (spinal muscles of New Zealand rabbits) to exclude the influence of conductive bone repair. The results showed that the addition of strontium could enhance the BMP-2-induced bone formation on implants in osteopenic rabbits to levels comparable to that in healthy rabbits. Secondarily, we observed more adipose tissue and cartilage tissue in osteopenic implants, suggesting the role of adipogenesis and chondrogenesis in osteopenia/osteoporosis. (C) 2015 Published by Elsevier Ltd. on behalf of Acta Materialia Inc.