Dengue viruses binding proteins from Aedes aegypti and Aedes polynesiensis salivary glands
Dengue viruses binding proteins from Aedes aegypti and Aedes polynesiensis salivary glands
复制标题
DOI：
10.1186/1743-422x-6-35
复制
发表时间：
2009-03-25
期刊：
影响因子：
4.8
通讯作者：
Cao-Lormeau, Van-Mai
中科院分区：
文献类型：
--
作者：
Cao-Lormeau, Van-Mai
登革热病毒（DENV）是登革热的病原体，通过具有传染性的蚊子吸收血液传播给人类宿主。媒介唾液腺的感染以及传染性唾液进一步注入人类宿主是 DENV 传播周期的关键事件。然而，DENV 进入蚊子唾液腺的分子机制尚未明确。另外，尽管对于其他媒介传播的病原体已证明昆虫唾液成分可能与宿主免疫剂相互作用并影响感染的建立，但蚊子唾液对人类 DENV 感染的作用的记录很少。为了识别在传播周期的这些关键步骤中可能与 DENV 相互作用的唾液腺分子，我们研究了两种蚊子的唾液腺提取物 (SGE) 中是否存在能够结合 DENV 的蛋白质。使用病毒覆盖蛋白结合测定，我们在埃及伊蚊 (L.) 和波利尼西亚伊蚊 (Marks) 的 SGE 中检测到了几种能够结合 DENV 的蛋白。目前的研究结果为鉴定介导登革热病毒附着或进入蚊子唾液腺的蛋白质以及可能在人类感染的最早阶段与病毒结合的唾液分泌蛋白质铺平了道路。目前的发现可能有助于确定抗登革热策略的新目标。
Dengue virus (DENV), the etiological agent of dengue fever, is transmitted to the human host during blood uptake by an infective mosquito. Infection of vector salivary glands and further injection of infectious saliva into the human host are key events of the DENV transmission cycle. However, the molecular mechanisms of DENV entry into the mosquito salivary glands have not been clearly identified. Otherwise, although it was demonstrated for other vector-transmitted pathogens that insect salivary components may interact with host immune agents and impact the establishment of infection, the role of mosquito saliva on DENV infection in human has been only poorly documented. To identify salivary gland molecules which might interact with DENV at these key steps of transmission cycle, we investigated the presence of proteins able to bind DENV in salivary gland extracts (SGE) from two mosquito species. Using virus overlay protein binding assay, we detected several proteins able to bind DENV in SGE from Aedes aegypti (L.) and Aedes polynesiensis (Marks). The present findings pave the way for the identification of proteins mediating DENV attachment or entry into mosquito salivary glands, and of saliva-secreted proteins those might be bound to the virus at the earliest step of human infection. The present findings might contribute to the identification of new targets for anti-dengue strategies.