Quantitative trait locus analysis of fungal disease resistance factors on a molecular map of grapevine
Quantitative trait locus analysis of fungal disease resistance factors on a molecular map of grapevine
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DOI：
10.1007/s00122-003-1445-3
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发表时间：
2004-02-01
影响因子：
5.4
通讯作者：
Zyprian, EM
中科院分区：
文献类型：
--
作者：
Fischer, BM;Salakhutdinov, I;Zyprian, EM
由来自“Regent”x“Lemberger”杂交的 153 个个体组成的全同胞 F1 群体被用来构建基于 429 个分子标记的遗传图谱。新培育的红葡萄品种“Regent”对真菌病害具有多重田间抗性，以多基因性状遗传，而“Lemberger”是传统的真菌敏感品种。后代对葡萄生单轴霉（Plasmopara viticola）和勾线霉（Uncinula necator）的抗性进行定量分离，这两种真菌病原体威胁着温带地区的葡萄栽培。采用双假测交策略在高统计严格性下构建两个亲本图谱进行连锁，以获得用于后续数量性状基因座（QTL）分析的稳健标记框架。总共绘制了 185 个扩增片段长度多态性、137 个随机扩增多态性 DNA、85 个单序列重复和 22 个序列特征扩增区域或切割的扩增多态性序列标记。这些图谱通过共显性或双杂合显性锚定标记进行比对。十二对同源连锁群可以整合成共有连锁群。在三个或四个生长季节对抗性表型和分离特征进行评分作为数量性状。对母体“Regent”图谱的三个连锁群上的特定区域进行区间映射，可重复定位与真菌疾病抗性相关的遗传因素。在连锁群 16 上鉴定了对 Uncinula necator 的抗性 QTL，在“Regent”的连锁群 9 和 10 上鉴定了对葡萄生单轴霉 (Plasmopara viticola) 的耐受性 QTL。确定了浆果成熟开始（“转色期”）、浆果大小和腋芽生长的其他 QTL。在两个红色品种的杂交中，浆果颜色被分离为一个简单的性状，并被绘制为形态标记。可以定位源自功能基因的六个标记。对葡萄树多基因真菌抗病性的剖析允许开发用于育种的标记辅助选择、遗传资源的表征和相应基因的分离。
A full-sibling F1 population comprising 153 individuals from the cross of 'Regent' x 'Lemberger' was employed to construct a genetic map based on 429 molecular markers. The newly-bred red grapevine variety 'Regent' has multiple field-resistance to fungal diseases inherited as polygenic traits, while 'Lemberger' is a traditional fungus-susceptible cultivar. The progeny segregate quantitatively for resistances to Plasmopara viticola and Uncinula necator, fungal pathogens that threaten viticulture in temperate areas. A double pseudo-testcross strategy was employed to construct the two parental maps under high statistical stringency for linkage to obtain a robust marker frame for subsequent quantitative trait locus (QTL) analysis. In total, 185 amplified fragment length polymorphism, 137 random amplified polymorphic DNA, 85 single sequence repeat and 22 sequence characterized amplified region or cleaved amplified polymorphic sequence markers were mapped. The maps were aligned by co-dominant or doubly heterozygous dominant anchor markers. Twelve pairs of homologous linkage groups could be integrated into consensus linkage groups. Resistance phenotypes and segregating characteristics were scored as quantitative traits in three or four growing seasons. Interval mapping reproducibly localized genetic factors that correlated with fungal disease resistances to specific regions on three linkage groups of the maternal 'Regent' map. A QTL for resistance to Uncinula necator was identified on linkage group 16, and QTLs for endurance to Plasmopara viticola on linkage groups 9 and 10 of 'Regent'. Additional QTLs for the onset of berry ripening ("veraison"), berry size and axillary shoot growth were identified. Berry color segregated as a simple trait in this cross of two red varieties and was mapped as a morphological marker. Six markers derived from functional genes could be localized. This dissection of polygenic fungus disease resistance in grapevine allows the development of marker-assisted selection for breeding, the characterization of genetic resources and the isolation of the corresponding genes.