Characterization of the mechanism of action of tachykinins in rat striatal cholinergic interneurons
Characterization of the mechanism of action of tachykinins in rat striatal cholinergic interneurons
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DOI：
10.1016/s0306-4522(98)00187-0
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发表时间：
1998-12-01
期刊：
影响因子：
3.3
通讯作者：
Lee, K
中科院分区：
文献类型：
--
作者：
Bell, MI;Richardson, PJ;Lee, K
采用全细胞记录技术研究了P物质使大鼠纹状体胆碱能中间神经元去极化的机制。在所有情况下，P物质的作用都被神经激肽(1)受体激动剂[Sar(9), Met(O-2)(11)] P物质模拟，并被神经激肽(1)受体拮抗剂SR 140333拮抗。[Sarg(9) Met(O-2)(11)] P物质通过诱导钙激活的- 60 mV内向电流使胆碱能中间神经元去极化。这种向内的电流被神经元内笼化GTP γ S的光解不可逆地增强，这暗示了一种g蛋白的参与。磷脂酶C抑制剂U-73122和在电极溶液中加入肌醇-1,4.5-三磷酸受体拮抗剂肝素可以抑制[Sarg(9) Met(O-2)(11)]物质p诱导的内向电流。这些发现表明，神经激肽(1)受体主要通过磷酸肌苷信号通路使大鼠纹状体胆碱能中间神经元去极化。(c) 1998 ibro。Elsevier Science Ltd.出版。
The mechanism by which substance P depolarizes cholinergic interneurons in the rat striatum was studied using whole-cell recording techniques. In all cases the effects of substance P were mimicked by the neurokinin(1) receptor agonist [Sar(9), Met(O-2)(11)] substance P and were antagonized by the neurokinin(1) receptor antagonist SR 140333. [Sarg(9) Met(O-2)(11)] substance P was found to depolarize cholinergic interneurons by the induction of a calcium-activated inward current at - 60 mV. This inward current was irreversibly potentiated by photolysis of caged GTP gamma S within neurons implicating the involvement of a G-protein. The [Sarg(9) Met(O-2)(11)] substance P-induced inward current was inhibited by the phospholipase C inhibitor U-73122, and by the inclusion of the inositol-1,4.5-triphosphate receptor antagonist heparin in the electrode solution.These findings suggest that neurokinin(1) receptors depolarize cholinergic interneurons in the rat striatum primarily through a phosphoinositide signalling pathway. (C) 1998 IBRO. Published by Elsevier Science Ltd.