Long-term maintenance of primary myogenic cultures on a reconstituted basement membrane.
Long-term maintenance of primary myogenic cultures on a reconstituted basement membrane.
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在重建的基底膜上长期维持原代生肌培养物。
DOI：
10.1007/bf02624469
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Yablonka-Reuveni,Z
中科院分区：
文献类型：
--
作者：
Hartley,RS;Yablonka-Reuveni,Z
我们描述了一种简单的技术，用于在Matrigel上长期保持高度收缩的鸡肌原性培养物，Matrigel是一种由从Engelbreth-Holm-Swarm小鼠肿瘤中提取的基底膜成分组成的凝胶。在Matrigel培养基上生长的培养物包括三维多层的圆柱形收缩肌管，这些肌管至少可以持续60天而不会脱落。基质增加了初始电镀效率，但不影响细胞增殖。与在明胶包覆的培养皿上培养相比，在Matrigel上培养的大规模分化延迟了1至2天。长期服用Matrigel也会导致新生儿和成人快速肌球蛋白重链亚型的表达增加。因此，在基质上培养细胞可以促进对体外肌生成后期事件的研究。
We describe a simple technique for maintaining highly contractile long-term chicken myogenic cultures on Matrigel, a gel composed of basement membrane components extracted from the Engelbreth-Holm-Swarm mouse tumor. Cultures grown on Matrigel consist of three-dimensional multilayers of cylindrical, contracting myotubes which endure for at least 60 d without myotube detachment. A Matrigel substrate increases the initial plating efficiency but does not effect cell proliferation. Large-scale differentiation in cultures maintained on Matrigel is delayed by 1 to 2 d, compared to cultures grown on gelatin-coated dishes. Long-term maintenance on Matrigel also results in increased expression of the neonatal and adult fast myosin heavy chain isoforms. Culturing of cells on a Matrigel substrate could thus facilitate the study of later events of in vitro myogenesis.