A multilocus analysis of Epicopeiidae (Lepidoptera, Geometroidea) provides new insights into their relationships and the evolutionary history of mimicry
A multilocus analysis of Epicopeiidae (Lepidoptera, Geometroidea) provides new insights into their relationships and the evolutionary history of mimicry
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对Epicopeiidae（鳞翅目、尺蛾总科）的多位点分析为它们的关系和拟态进化史提供了新的见解
DOI：
10.1016/j.ympev.2020.106847
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发表时间：
2020
影响因子：
4.1
通讯作者：
Zhang Peng
中科院分区：
文献类型：
--
作者：
Zhang Yuan;Huang SiYao;Liang Dan;Wang HouShuai;Zhang Peng
Epicopeiidae是一小群白天飞行的飞蛾，以模仿许多不同的蝴蝶和飞蛾而闻名。到目前为止，仍然缺乏一个可靠的系统发育框架，这是必要的，我们了解他们的拟态进化过程。在这项研究中，我们测序了94个核蛋白编码标记的56 epicopeiid样本和11个外类群，涵盖了所有10个属的Epicopeiidae。我们使用自制的PCR生成的诱饵来捕获靶序列，这使我们能够利用传统PCR +桑格测序难以处理的旧的和干燥的标本。在DNA和蛋白质水平上对新获得的数据集（86，388 bp）进行的最大似然和贝叶斯分析产生了具有强有力支持的相同同源性。非拟态属Deuvei是Epicopeia科其他属的姐妹群，Epicopeia和Nossa不是单系的，这两个属嵌套在一起形成一个分支。我们还估计了Epicopeiidae的分化时间，发现它们的最初分化发生在大约4100万年前的始新世。拟态型的祖先状态重建结果表明，拟蛾科的最后共同祖先是非拟态的，拟蛾科的拟态型是最先进化的。总之，我们的工作提供了一个全面的和强大的时间校准的Epicopeiidae，提供了一个健全的框架，修改其分类和解释字符的进化。
The family Epicopeiidae is a small group of day-flying moths, known for mimicking many different groups of butterflies and moths. So far, there still lacks a reliable phylogenetic framework of Epicopeiidae that is necessary to our understanding of the evolutionary process of their mimicry. In this study, we sequenced 94 nuclear protein-coding markers for 56 epicopeiid samples and 11 outgroups, covering all ten genera of Epicopeiidae. We used homemade PCR-generated baits to capture target sequences, which allowed us to utilize old and dried specimens that were difficult to handle by conventional PCR + Sanger sequencing. Maximum likelihood and Bayesian analyses of the newly obtained dataset (86,388 bp) at both DNA and protein levels produced identical phylogenies with strong support. The non-mimicry genusDeuveiais the sister group of other epicopeiid genera.EpicopeiaandNossaare not monophyletic, and these two genera nest together to form a clade. We also estimated divergence times of Epicopeiidae and found that their initial diversification happened in Eocene about 41 million years ago. The ancestral state reconstruction of mimicry type for this family suggested that the last common ancestor of epicopeiid moths is non-mimetic, and the Riodinidae-mimicry type evolved first. In summary, our work provides a comprehensive and robust time-calibrated phylogeny of Epicopeiidae that provides a sound framework for revising their classification and interpreting character evolution.