The long-term effects of ovariectomy on bone metabolism in sheep
The long-term effects of ovariectomy on bone metabolism in sheep
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DOI：
10.1007/s00774-006-0724-x
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发表时间：
2007-01-01
影响因子：
3.3
通讯作者：
Egermann, Marcus
中科院分区：
文献类型：
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作者：
Sigrist, Ilonka M.;Gerhardt, Christian;Egermann, Marcus
骨质疏松症和相关骨折是主要的公共卫生问题，因此需要适当的大型动物模型来进一步了解这种疾病。尽管绵羊似乎是研究雌激素消耗引起的骨质流失的理想模型，但有关卵巢切除术对绵羊骨质的长期影响的数据有限。本研究的目的是观察 18 个月内卵巢切除引起的绵羊骨量、结构和代谢的变化。六只母羊被切除卵巢（OVX），并通过分析骨矿物质密度、小梁结构、骨形成和吸收的生化标志物以及血浆雌激素水平与年龄匹配的对照组进行比较。手术后前 4 个月发生骨质流失（13%，P < 0.01），然后在研究的剩余时间内稳定并恢复到 OVX 前的水平。小梁结构也发生了改变，并趋向于骨质减少，并恢复到基线值。卵巢切除术后 6 个月内，骨形成和吸收标志物升高，此后水平恢复至基线值。尽管卵巢切除术后雌二醇测量值明显下降，但 6 个月后水平恢复正常。因此，卵巢切除术对绵羊骨代谢的不利影响似乎是可逆的，术后6个月内即可恢复正常的骨参数。这些数据似乎表明绵羊不是人类绝经后骨质疏松症的合适模型。
Osteoporosis and associated fractures are major public health concerns, and as such require appropriate large animal models to further our understanding of this disease. Although sheep appear to be an ideal model with which to study bone loss caused by estrogen depletion, limited data are available concerning the long-term effect of ovariectomy on bone in sheep. The goal of the present study was to observe the ovariectomy-induced changes in bone mass, structure, and metabolism in sheep over a period of 18 months. Six ewes were ovariectomized (OVX) and compared to an age-matched control group by analyzing bone mineral density, trabecular structure, biochemical markers of bone formation and resorption, and plasma estrogen levels. Bone loss (13%, P < 0.01) occurred during the first 4 months after surgery, then stabilized and returned to pre-OVX levels for the remainder of the study. Trabecular architecture was also altered and tended toward osteopenia with recovery to baseline values. Markers of bone formation and resorption were elevated up to 6 months postovariectomy, after which time levels returned to baseline values. Although estradiol measurements demonstrated a clear decline following surgical ovariectomy, levels returned to normal after 6 months. Therefore, the detrimental effect of ovariectomy on sheep bone metabolism seems to be reversible, with normal bone parameters being reestablished within 6 months after surgery. These data seem to indicate that the sheep is not an appropriate model for human postmenopausal osteoporosis.