Phospholipase C-gamma contains introns shared by src homology 2 domains in many unrelated proteins.
Phospholipase C-gamma contains introns shared by src homology 2 domains in many unrelated proteins.
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磷脂酶 C-gamma 包含许多不相关蛋白质中 src 同源 2 结构域共享的内含子。
DOI：
10.1093/genetics/164.2.433
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发表时间：
2003
期刊：
影响因子：
3.3
通讯作者：
Thackeray,JustinR
中科院分区：
文献类型：
--
作者：
Manning,CharleneM;Mathews,WendyR;Fico,LeahP;Thackeray,JustinR
许多具有新功能的蛋白质是在后生动物辐射前后通过外显子改组而产生的。磷脂酶C-γ(PLC-γ)是此时出现的典型蛋白质，它包含几个可能起源于其他地方的不同模块。为了深入了解小翅(Sl)编码的果蝇plc-γ的进化和结构与功能的关系，我们克隆了果蝇plc-γ和D的plc-γ同源序列。并将它们的基因结构和预测的氨基酸序列与其他动物的PLC-γ同源物进行了比较。PLC-γ在整个过程中都被很好地保守，尽管结构差异表明酪氨酸磷酸化在酶激活中的作用在脊椎动物和无脊椎动物中是不同的。内含子位置的比较表明，在无脊椎动物进化过程中发生了广泛的内含子丢失，同时也揭示了在N端和C端的PLC-γSH2域中都存在保守的内含子，这些内含子存在于许多其他基因的SH2域中。这些和其他保守的SH2内含子表明，PLC-γ中的SH2结构域来自于一个祖先结构域，该结构域在动物进化过程中不仅被改组到PLC-γ中，还被改组到许多其他无关的基因中。
Many proteins with novel functions were created by exon shuffling around the time of the metazoan radiation. Phospholipase C-γ (PLC-γ) is typical of proteins that appeared at this time, containing several different modules that probably originated elsewhere. To gain insight into both PLC-γ evolution and structure-function relationships within the Drosophila PLC-γ encoded bysmall wing(sl), we cloned and sequenced the PLC-γ homologs fromDrosophila pseudoobscuraandD. virilisand compared their gene structure and predicted amino acid sequences with PLC-γ homologs in other animals. PLC-γ has been well conserved throughout, although structural differences suggest that the role of tyrosine phosphorylation in enzyme activation differs between vertebrates and invertebrates. Comparison of intron positions demonstrates that extensive intron loss has occurred during invertebrate evolution and also reveals the presence of conserved introns in both the N- and C-terminal PLC-γ SH2 domains that are present in SH2 domains in many other genes. These and other conserved SH2 introns suggest that the SH2 domains in PLC-γ are derived from an ancestral domain that was shuffled not only into PLC-γ, but also into many other unrelated genes during animal evolution.