Real-Time Monitoring of Glucose Export from Isolated Chloroplasts Using an Organic Electrochemical Transistor
Real-Time Monitoring of Glucose Export from Isolated Chloroplasts Using an Organic Electrochemical Transistor
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DOI：
10.1002/admt.201900262
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发表时间：
2020-03-01
影响因子：
6.8
通讯作者：
Stavrinidou, Eleni
中科院分区：
文献类型：
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作者：
Diacci, Chiara;Lee, Jee Woong;Stavrinidou, Eleni
基于有机电化学晶体管（OECT）的生物传感器是一种非常有吸引力的生物过程实时监测装置。OECT的通道和电解质之间的直接耦合使得能够与生物环境密切接触，同时带来信号放大和快速传感器响应时间。到目前为止，这些设备主要应用于哺乳动物系统;细胞或体液的诊断和各种健康状况监测技术的发展。然而，没有报道直接检测来自细胞或细胞器的生物分子。在这里，OECT葡萄糖传感器应用于叶绿体，这是负责光合作用的光化学能转换的植物细胞器，报告。在两个不同的代谢阶段的叶绿体葡萄糖输出的实时监测证明和1分钟的时间分辨率的转移动力学进行量化，从而达到监测动力学是一个数量级优于传统的方法。
Biosensors based on organic electrochemical transistors (OECT) are attractive devices for real-time monitoring of biological processes. The direct coupling between the channel of the OECT and the electrolyte enables intimate interfacing with biological environments at the same time bringing signal amplification and fast sensor response times. So far, these devices are mainly applied to mammalian systems; cells or body fluids for the development of diagnostics and various health status monitoring technology. Yet, no direct detection of biomolecules from cells or organelles is reported. Here, an OECT glucose sensor applied to chloroplasts, which are the plant organelles responsible for the light-to-chemical energy conversion of the photosynthesis, is reported. Real-time monitoring of glucose export from chloroplasts in two distinct metabolic phases is demonstrated and the transfer dynamics with a time resolution of 1 min is quantified, thus reaching monitoring dynamics being an order of magnitude better than conventional methods.