The chemistry and biology of unusual DNA structures adopted by oligopurine · oligopyrimidine sequences
The chemistry and biology of unusual DNA structures adopted by oligopurine · oligopyrimidine sequences
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DOI：
10.1096/fasebj.2.14.3053307
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发表时间：
1988-11
期刊：
影响因子：
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通讯作者：
R. Wells;D. Collier;J. Hanvey;M. Shimizu;F. Wohlrab
中科院分区：
文献类型：
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作者：
R. Wells;D. Collier;J. Hanvey;M. Shimizu;F. Wohlrab
在一条链（pur·pyr序列）中主要含有嘌呤的片段中发现了一个不寻常的DNA结构家族。这些序列在真核生物DNA中被广泛使用，并且在基因和重组热点附近被定位。当克隆到重组质粒时，许多pur·pyr序列对化学和酶探针有反应，这些探针通常是单链DNA特异性的。G's和A's的镜像重复采用分子内三联体。类似序列采用的其他非B DNA结构仍有待完全澄清，但可能是一个相关构象家族。很可能这些非正统结构在真核生物基因组的功能中起着重要的作用。——威尔斯，r.d.；科利尔博士；汉维，j.c.；清水,m;王志强，王志强，王志强，等。1988.
A family of unusual DNA structures has been discovered in segments with predominantly purines in one strand (pur · pyr sequences). These sequences are overrepresented in eukaryotic DNA and have been mapped near genes and recombination hot spots. When cloned into recombinant plasmids, many pur · pyr sequences are reactive to chemical and enzymic probes that are generally specific for single‐stranded DNA. An intramolecular triplex is adopted by mirror repeats of G's and A's. Other non‐B DNA structures adopted by similar sequences remain to be fully clarified but may be a family of related conformations. It is likely that these unorthodox structures play an important role in the function of the eukaryotic genome.— Wells, R. D.; Collier, D A.; Hanvey, J. C.; Shimizu, M.; Wohlrab, F. FASEB J. 2: 2939‐2949; 1988.