ALTERED EPITHELIAL BASEMENT-MEMBRANE INTERACTIONS IN DIABETIC CORNEAS
ALTERED EPITHELIAL BASEMENT-MEMBRANE INTERACTIONS IN DIABETIC CORNEAS
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DOI：
10.1001/archopht.1992.01080160115045
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发表时间：
1992-04-01
影响因子：
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通讯作者：
GIPSON, IK
中科院分区：
文献类型：
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作者：
AZAR, DT;SPURRMICHAUD, SJ;GIPSON, IK
糖尿病患者细胞外基质的形态改变包括基底膜增厚，在角膜上皮细胞中，半桥粒占据的基底膜面积减少。为了确定糖尿病患者异常的基底膜是否改变了上皮对半桥粒形成部位的识别，以及糖尿病上皮细胞是否能够识别正常基底膜上的半桥粒形成部位，我们使用糖尿病角膜和对照角膜，在体外将上皮片和角膜基质与剥离的基底膜重组。在正常基质上的糖尿病上皮细胞重组中，半桥粒占据基底膜的面积为10.7%，显著小于对照重组的19.5%。同样，在糖尿病间质中，这一值(8.6%)显著低于对照组。这些数据可能表明糖尿病患者细胞-细胞外基质相互作用发生改变。
Morphologic alterations of the extracellular matrix in diabetes include thickening of the basement membrane and, as evidenced in the corneal epithelium, a decrease in the area of the basal cell membrane occupied by hemidesmosomes. To determine if the abnormal basement membrane in diabetes has altered epithelial recognition of sites for hemidesmosome formation and if diabetic epithelium can recognize sites for hemidesmosome formation on normal basement membrane, we recombined epithelial sheets and corneal stromas with denuded basement membranes in vitro using diabetic and control corneas. In recombinations of diabetic epithelium on normal stromas, the area of the basal cell membrane occupied by hemidesmosomes was 10.7%, significantly less than control recombinations (19.5%). Similarly, in diabetic stromas, the value (8.6%) was significantly decreased from control. These data may indicate altered cell-extracellular matrix interactions in diabetes.