Hypoosmotic stress induced functional alternations of intestinal barrier integrity, inflammatory reactions, and neurotransmission along gut‑brain axis in the yellowfin seabream (Acanthopagrus latus)
Hypoosmotic stress induced functional alternations of intestinal barrier integrity, inflammatory reactions, and neurotransmission along gut‑brain axis in the yellowfin seabream (Acanthopagrus latus)
复制标题
低渗应激引起黄鳍鲷（Acanthopagrus latus）肠屏障完整性、炎症反应和肠脑轴神经传递的功能改变
DOI：
10.1007/s10695-021-01011-x
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发表时间：
2021
影响因子：
2.9
通讯作者：
Jianguo Lu
中科院分区：
文献类型：
--
作者：
Genmei Lin;Shizhu Li;Dong Gao;Junrou Huang;Jianguo Lu
肠脑轴在多种代谢调节过程中发挥重要作用，但关于其对鱼类环境应激反应的研究仍然有限。在本研究中，我们对暴露于不同盐度环境（淡水：0 ppt，低咸水：3 ppt，微咸水：6 ppt）的黄鳍海鲷（Acanthopagrus latus）进行了转录组测序分析和酶联免疫吸附试验（ELISA）。通过转录组分析，分别鉴定出707个和1477个基因在淡水和微咸水处理下的大脑和肠道中存在差异表达基因（deg）。脑deg显著富集为一组与信号转导相关的基因本体术语和京都基因与基因组百科全书（KEGG）通路，其中大部分被下调。肠道DEGs富集为与神经传递相关的KEGG通路色氨酸代谢，下调的DEGs富集为KEGG通路局灶黏附。ELISA通过测定紧密连接蛋白ZO-2、白细胞介素1β和血清素的浓度，证实了大脑和肠道在不同治疗过程中的显著生理反应。在低渗应激下，肠-脑轴的功能通过肠屏障完整性受损、肠-脑神经传递紊乱和组织损伤性炎症反应而改变。本研究确定了黄鳍鱼在低渗应激下肠-脑轴表达差异显著的候选基因，为理解硬骨鱼肠-脑轴的潜在渗透调节机制提供了线索。
The gut-brain axis plays a major role in multiple metabolic regulation processes, but studies regarding its responses to environmental stress in fish are still limited. In this study, we performed transcriptome sequencing analysis and enzyme-linked immunosorbent assay (ELISA) in yellowfin seabream (Acanthopagrus latus) exposed to environments with different water salinity (freshwater: 0 ppt; low-saline water: 3 ppt; brackish water: 6 ppt). According to transcriptome analysis, 707 and 1477 genes were identified as differentially expressed genes (DEGs) between freshwater and brackish water treatments in the brain and gut, respectively. Brain DEGs were significantly enriched into a set of Gene Ontology terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways associated with signal transduction, most of which were downregulated. Gut DEGs were enriched into a neurotransmission-relevant KEGG pathway tryptophan metabolism, and the downregulated DEGs were enriched into the KEGG pathway focal adhesion. ELISA demonstrated significant physiological responses of the brain and gut across treatments, as determined by the concentrations of tight junction protein ZO-2, interleukin 1β, and serotonin. Under hypoosmotic stress, the functions of the gut-brain axis are altered via impairment of intestinal barrier integrity, by disturbance of gut-brain neurotransmission, and through tissue-damaging inflammatory reactions. Our work identified candidate genes which showed significantly differential expression in the gut-brain axis when yellowfin seabream encountered hypoosmotic stress, which could shed lights on the understanding of the potential osmotic regulation mechanisms of the gut-brain axis in teleost.