Immunosuppressive functions of melanoma cell-derived exosomes in plasma of melanoma patients.
Immunosuppressive functions of melanoma cell-derived exosomes in plasma of melanoma patients.
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DOI：
10.3389/fcell.2022.1080925
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发表时间：
2022
影响因子：
5.5
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中科院分区：
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肿瘤来源的外体(TeX)是存在于癌症患者所有体液中的小细胞外小泡(SEV)的一个子集。在转移性黑色素瘤患者的血浆中，由黑色素瘤细胞产生的外切体数量增加，称为MTEX。为了研究MTEX在黑色素瘤进展中的作用，采用基于免疫亲和力的方法从血浆总外切体中分离MTEX。MTEX表面修饰了多种检查点抑制蛋白，与免疫受体细胞共同孵育后，MTEX抑制了这些细胞的抗肿瘤功能。MTEX是黑色素瘤免疫抑制的主要机制，因此可能在促进黑色素瘤进展中发挥作用。
Tumor-derived exosomes (TEX) are a subset of small extracellular vesicles (sEV) present in all body fluids of patients with cancer. In plasma of patients with metastatic melanoma, numbers of exosomes produced by melanoma cells called MTEX are elevated. To study the role of MTEX in melanoma progression, immunoaffinity-based separation of MTEX from total plasma exosomes was performed. The surface of MTEX was decorated by various checkpoint inhibitory proteins, and upon coincubation with immune recipient cells, MTEX suppressed anti-tumor functions of these cells. MTEX emerge as a major mechanism of immune suppression in melanoma and thus might play a role in promoting melanoma progression.