Modulation of the Phosphate-Deficient Responses by MicroRNA156 and its Targeted SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 3 in Arabidopsis
Modulation of the Phosphate-Deficient Responses by MicroRNA156 and its Targeted SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 3 in Arabidopsis
复制标题
MicroRNA156 及其靶向鳞状启动子结合蛋白样 3 对拟南芥中磷酸盐缺乏反应的调节
DOI：
10.1093/pcp/pcv197
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发表时间：
2016-01-01
影响因子：
4.9
通讯作者：
Song, Chun-Peng
中科院分区：
文献类型：
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作者：
Lei, Kai-Jian;Lin, Ya-Ming;Song, Chun-Peng
MicroRNA156(MiR156)调控的Squamosa启动子结合蛋白样蛋白(SPL)参与了生长、发育和代谢等多种生物学过程。在此，我们报道了拟南芥miR156和SPL3作为调节因子在磷缺乏反应中发挥重要作用。在PI饥饿过程中，miR156被诱导表达，而SPL3的表达被抑制。在磷缺乏条件下，35S：MIM156(通过靶标拟态)转基因植株根际酸化程度降低，花青素积累减少。与野生型(Col-0生态型)相比，35S：MIM156拟南芥植株的PI含量和吸收增加。35S：rSPL3植株的花青素积累和PI含量表型与35S：MIM156相似。染色质免疫沉淀和电泳迁移率改变分析表明，SPL3蛋白直接与PLDZ2、Pht1；5和miR399f启动子中的GTAC基序结合。在35S：MIM156和35S：rSPL3植株中，一些PI饥饿诱导基因的表达增加，包括高亲和力的PI转运蛋白、MT4/TPS1类基因和磷酸酶。综上所述，我们的结果表明，miR156-SPL3-Pht1；5(-PLDZ2和miR399f)通路构成了PI缺乏诱导的拟南芥调控机制的一个组成部分。
The microRNA156 (miR156)-modulated SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) is involved in diverse biological processes that include growth, development and metabolism. Here, we report that the Arabidopsis miR156 and SPL3 as regulators play important roles in phosphate (Pi) deficiency response. MiR156 was induced during Pi starvation whereas SPL3 expression was repressed. Phenotypes of reduced rhizosphere acidification and decreased anthocyanin accumulation were observed in 35S:MIM156 (via target mimicry) transgenic plants under Pi deficiency. The content and uptake of Pi in 35S:MIM156 Arabidopsis plants were increased compared with wild-type (Col-0 ecotype) plants. 35S:rSPL3 seedlings showed similar anthocyanin accumulation and Pi content phenotypes to those of 35S:MIM156 plants. Chromatin immunoprecipitation and an electrophoretic mobility shift assay indicated that the SPL3 protein directly bound to GTAC motifs in the PLDZ2, Pht1;5 and miR399f promoters. The expression of several Pi starvation-induced genes was increased in 35S:MIM156 and 35S:rSPL3 plants, including high-affinity Pi transporters, Mt4/TPS1-like genes and phosphatases. Collectively, our results suggest that the miR156-SPL3-Pht1; 5 (-PLDZ2 and miR399f) pathways constitute a component of the Pi deficiency-induced regulatory mechanism of Arabidopsis.