Electrochemical enzyme immunoassay using sequential saturation technique in a 20-μl capillary: digoxin as a model analyte
Electrochemical enzyme immunoassay using sequential saturation technique in a 20-μl capillary: digoxin as a model analyte
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在 20 µl 毛细管中使用顺序饱和技术进行电化学酶免疫分析：地高辛作为模型分析物
DOI：
10.1016/0003-2670(93)e0612-b
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发表时间：
1994
影响因子：
6.2
通讯作者：
Peter T. Kissinger
中科院分区：
文献类型：
--
作者：
N. Kaneki;Yan Xu;A. Kumari;H. Halsall;W. Heineman;Peter T. Kissinger
建立了地高辛的流动注射顺序饱和毛细管酶免疫分析法。玻璃毛细管（10 cm × 0.53 mm内径）与地高辛抗体固定的免疫反应器。酶促反应的产物。4-氨基苯酚，检测安培。地高辛和标记地高辛与固定化地高辛抗体的结合反应分别在2和10 min内完成。地高辛在20 μl样品中测定，检测限为10 pg ml−1（200 fg或260阿托摩尔），范围为3个数量级。
Capillary enzyme immunoassay with flow-injection analysis for digoxin using the sequential saturation technique has been developed. Glass capillary tubes (10 cm × 0.53 mm i.d.) with immobilized digoxin antibody were used as the immunoassay reactor. The product of enzymatic reaction. 4-aminophenol, was detected amperometrically. The digoxin and the labeled digoxin binding reaction with the immobilized digoxin antibody were completed in 2 and 10 min, respectively. Digoxin was determined in a 20-μl sample with a detection limit of 10 pg ml−1(200 fg or 260 attomoles) and a 3 orders of magnitude range.
基于液相色谱的酶放大免疫学测定，在 Zeptomole 水平的熔融二氧化硅毛细管中进行。
影响因子：
7.4
作者：
deFrutos,M;Paliwal,SK;Regnier,FE
通讯作者：
Regnier,FE
核糖核酸聚合酶的单核苷酸和二核苷酸光亲和探针的合成。
影响因子：
2.9
作者：
DeRiemer,LH;Meares,CF
通讯作者：
Meares,CF