Tissue distribution study of columbianadin and its active metabolite columbianetin in rats
Tissue distribution study of columbianadin and its active metabolite columbianetin in rats
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DOI：
10.1002/bmc.3543
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发表时间：
2016-02-01
影响因子：
1.8
通讯作者：
Yang, Xiu-Wei
中科院分区：
文献类型：
--
作者：
Zhang, You-Bo;Yang, Xiu-Wei
Columbianadin，当归根的主要生物活性成分之一。 f.前期研究已发现biserrata Shan et Yuan具有明显的药理作用。本研究建立并验证了一种有效且灵敏的反相高效液相色谱（RP-HPLC）方法，用于测定大鼠组织样品中的哥伦比亚铜定（CBN）及其活性代谢物哥伦比亚铜定（CBT）。样品分离采用 RP-HPLC 柱，流动相为 MeOH-H2O（75:25，v/v），流速为 1.0mL/min。在波长325nm处测量样品的UV吸光度。 CBN的校准曲线在脑、睾丸和肌肉0.5-20μg/g范围内呈线性，胃和肠在1.0-10.0μg/g范围内，心、肝、脾、肺和肾在0.2-20.0μg/g范围内呈线性。胃和肠的CBT校准曲线在0.5-25μg/g范围内呈线性，心、肝、脾、肺和肾的CBT校准曲线在0.1-10.0μg/g范围内呈线性。该分析方法成功应用于大鼠静脉注射CBN后CBN和CBT的组织分布研究。这项研究的结果表明，静脉注射后，在所有选定的组织中都可以检测到 CBN。行政。 CBN 迅速分布到大鼠组织中，并且在大多数检测到的组织中可以代谢为 CBT。在检测到的组织中，心脏对CBN的摄取量最高，这表明心脏可能是CBN的主要靶组织之一。消化系统中的 CBT 浓度明显高于其他检测组织。这项研究提供的信息对于了解 CBN 和 CBT 访问不同组织的能力非常有用。版权所有 (c) 2015 约翰·威利父子有限公司
Columbianadin, one of the main bioactive constituents of the roots of Angelica pubescens Maxim. f. biserrata Shan et Yuan, has been found to possess obvious pharmacological effects in previous studies. In this study, a valid and sensitive reverse-phase high-performance liquid chromatography (RP-HPLC) method was established and validated for the determination of columbianadin (CBN) and its active metabolite columbianetin (CBT) in rat tissue samples. Sample separation was performed on an RP-HPLC column using a mobile phase of MeOH-H2O (75:25, v/v) at a flow rate of 1.0mL/min. The UV absorbance of the samples was measured at the wavelength 325nm. The calibration curves for CBN were linear over the ranges of 0.5-20 mu g/g for brain, testes and muscle, 1.0-10.0 mu g/g for stomach and intestine, and 0.2-20.0 mu g/g for heart, liver, spleen, lung and kidney. The calibration curves for CBT were linear over the ranges of 0.5-25 mu g/g for stomach and intestine, and 0.1-10.0 mu g/g for heart, liver, spleen, lung and kidney. The analysis method was successfully applied to a tissue distribution study of CBN and CBT after intravenous administration of CBN to rats. The results of this study indicated that CBN could be detected in all of the selected tissues after i.v. administration. CBN was distributed to rat tissues rapidly and could be metabolized to CBT in most detected tissues. Of the detected tissues, heart had the highest uptake of CBN, which suggested that heart might be one of the main target tissues of CBN. Concentrations of CBT were obviously higher in the digestive system than in other assayed tissues. The information provided by this research is very useful for gaining knowledge of the capacities of CBN and CBT to access different tissues. Copyright (c) 2015 John Wiley & Sons, Ltd.