Design considerations for proteomic reference materials
Design considerations for proteomic reference materials
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DOI：
10.1002/pmic.201000242
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发表时间：
2010-12-01
期刊：
影响因子：
3.4
通讯作者：
Bunk, David M.
中科院分区：
文献类型：
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作者：
Bunk, David M.
为了提高基于MS的蛋白质组学测量的可重复性、可比性和准确性，国际上已经做出了相当大的努力来开发适当的参考材料。尽管大多数参考材料被开发用于支持常规测定的测量质量，但是用于多样化和不断变化的研究领域（例如蛋白质组学）的参考材料的开发代表了独特的挑战。典型蛋白质组学样品的共同特征，由于该领域的多样性和不断变化的性质，必须考虑支撑蛋白质组学的计量学。然后可以围绕共同方面设计参考材料，以便产生具有最广泛适用性的参考材料。需要参考材料来支持定性和定量蛋白质组学测量，涉及不同的设计考虑需要建立共识和经验证的统计方法来描述定性测量（如蛋白质鉴定）的置信度在蛋白质组学参考物质设计中还需要考虑测量偏倚的常见来源
In order to Improve the repeatability comparabihty and accuracy of MS based proteomic measurements there has been considerable international effort to develop appropriate reference materials Although the majority of reference materials are developed to support measurement quality of routine assays the development of reference materials for a diverse and changing research field such as proteomics represents unique challenges In order to define common measurement components and common features of typical proteomic samples, the metrology underpinning proteomics must be considered due to the diversity and changing nature of the field Reference materials can then be designed around common aspects in order to produce reference materials with the broadest applicability Reference materials are needed to support both qualitative and quantitative proteomic measurements, involving different design considerations Consensus and validated statistical approaches to describe the confidence in qualitative measurement such as protein identification needs to be established Common sources of measurement bias also need to be considered in proteomic reference material design