Isoprenylation of Monomeric GTPases in Human Trabecular Meshwork Cells.
Isoprenylation of Monomeric GTPases in Human Trabecular Meshwork Cells.
复制标题
人小梁网细胞中单体 GTP 酶的异戊二烯化。
DOI：
10.1007/978-1-4939-6996-8_18
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
StubbsJr,EvanB
中科院分区：
文献类型：
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作者：
StubbsJr,EvanB
小单体gtp酶，包括那些属于Rho家族的gtp酶，调节多种细胞内信号通路，影响囊泡运输/运输、内吞作用、细胞周期进程、细胞收缩性和应力纤维或局灶粘连的形成。新合成的小单体gtpase的功能激活是通过一个多步骤的翻译后过程来促进的，这个过程包括转移酶催化的法尼基或香叶基异戊二烯类在一个独特的羧基末端CaaX基序中保守的半胱氨酸残基上的添加。在这里，使用完善的和广泛可用的当代方法，详细的方案，其中半定量评估翻译后异戊二烯化在人类小梁网细胞的功能后果描述。我们介绍了异戊二烯化本身是哺乳动物Rho GTPase表达和转换的关键调节因子的概念。
Small monomeric GTPases, including those belonging to the Rho family, regulate a diverse array of intracellular signaling pathways which affect vesicle transport/trafficking, endocytosis, cell cycle progression, cell contractility, and formation of stress fibers or focal adhesions. Functional activation of newly synthesized small monomeric GTPases is facilitated by a multistep post-translational process involving transferase-catalyzed addition of farnesyl or geranylgeranyl isoprenoids to conserved cysteine residues within a unique carboxy terminal CaaX motif. Here, using well-established and widely available contemporary methodologies, detailed protocols by which to semi-quantitatively evaluate the functional consequence of post-translational isoprenylation in human trabecular meshwork cells are described. We introduce the concept that isoprenylation alone is itself a key regulator of mammalian Rho GTPase expression and turnover.