EPSTEIN-BARR NUCLEAR ANTIGEN-1 MEDIATES A DNA LOOP WITHIN THE LATENT REPLICATION ORIGIN OF EPSTEIN-BARR-VIRUS
EPSTEIN-BARR NUCLEAR ANTIGEN-1 MEDIATES A DNA LOOP WITHIN THE LATENT REPLICATION ORIGIN OF EPSTEIN-BARR-VIRUS
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DOI：
10.1073/pnas.88.23.10875
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发表时间：
1991-12-01
影响因子：
11.1
通讯作者：
ODONNELL, M
中科院分区：
文献类型：
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作者：
FRAPPIER, L;ODONNELL, M
EB病毒编码的核抗原1（EBNA-1）结合并激活病毒潜在的DNA复制起点oriP。我们已经使用电子显微镜检查EBNA-1到oriP上的组装。oriP区域由两个基本元件组成，这两个基本元件被大约1对DNA内切酶分开。一个元件含有20个串联EBNA-1结合位点[称为重复序列家族（FR）]，并用于激活二分体对称（DS）元件处的复制起始，该二分体对称（DS）元件含有4个EBNA-1结合位点。将杆状病毒中产生的均一EBNA-1（bEBNA-1）滴加到oriP DNA上，显示bEBNA-1在oriP上的组装是有序的。在低浓度下，bEBNA-1仅位于FR元件上。随着bEBNA-1水平的升高，FR和DS元件之间的环成为最普遍的DNA-蛋白质复合物。这些数据表明，蛋白质介导的DNA成环可能在激活EB病毒的潜伏期复制中发挥作用。
Epstein-Barr virus-encoded nuclear antigen 1 (EBNA-1) binds and activates the viral latent origin of DNA replication, oriP. We have used electron microscopy to examine the assembly of EBNA-1 onto oriP. The oriP region consists of two essential elements separated by approximately 1 kilobase pair of DNA. One element contains 20 tandom EBNA-1 binding sites [called the family of repeats (FR)] and serves to activate initiation of replication at the dyad symmetry (DS) element, which contains 4 EBNA-1 binding sites. Titration of homogeneous EBNA-1 produced in baculovirus (bEBNA-1) onto oriP DNA showed an order to the assembly of bEBNA-1 onto oriP. At low concentrations, bEBNA-1 was located exclusively on the FR element. As the level of bEBNA-1 was raised, a loop between the FR and DS elements became the most prevalent DNA-protein complex. These data suggest protein-mediated DNA looping may play a role in activating latent-phase replication of the Epstein-Barr virus.