Mild sensory stimulation reestablishes cortical function during the acute phase of ischemia.
Mild sensory stimulation reestablishes cortical function during the acute phase of ischemia.
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DOI：
10.1523/jneurosci.1741-11.2011
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发表时间：
2011-08-10
期刊：
影响因子：
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通讯作者：
Frostig RD
中科院分区：
文献类型：
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作者：
Lay CC;Davis MF;Chen-Bee CH;Frostig RD
当在永久性大脑中动脉闭塞（pMCAO）后1小时内（大多数情况下为2小时内）给予轻度感觉刺激（间歇性单须刺激）时，根据在缺血性卒中啮齿动物模型中pMCAO后24小时采用多种技术进行的评估，显示出完全的神经保护作用。然而，刺激治疗对缺血皮质的急性影响尚未报道。在这里，我们表征皮质功能和灌注在120分钟的晶须刺激期间，在四个实验组与治疗开始0，1，2小时（保护组）或3小时后pMCAO（未保护组）使用多种技术。根据功能成像，诱发的触须功能表征逐渐恢复到基线水平开始与治疗开始，并在治疗期间完成。诱发的神经元活动和缺血区的再灌注也显示出在保护动物中逐渐恢复。令人惊讶的是，无论给药开始时间如何，在所有受保护动物中均观察到对给药的响应相似的恢复曲线。非刺激的pMCAO对照组数据表明再灌注不是自发的。这使得在pMCAO后2小时刺激的大多数动物中观察到的完全保护甚至更令人惊讶，因为这些动物尽管已经处于这种严重缺血状态整整两个小时而恢复。总之，当在pMCAO后2小时窗口内递送时，晶须刺激治疗引发再灌注和皮质功能的逐渐恢复，其在治疗期内完成或接近完成。
When delivered within 1 and in most cases 2 hours of permanent middle cerebral artery occlusion (pMCAO), mild sensory stimulation (intermittent single whisker stimulation) was shown to be completely neuroprotective according to assessment with multiple techniques 24 hours after pMCAO in a rodent model of ischemic stroke. The acute effect of stimulation treatment on the ischemic cortex however, had yet to be reported. Here we characterize cortical function and perfusion during the 120 minute whisker stimulation period in four experimental groups with treatment initiated 0, 1, 2 hours (protected groups) or 3 hours post-pMCAO (unprotected group) using multiple techniques. According to functional imaging, a gradual return of evoked whisker functional representation to baseline levels was initiated with treatment onset and completed within the treatment period. Evoked neuronal activity and reperfusion to the ischemic area also showed a gradual recovery in protected animals. Surprisingly, a similar recovery profile was observed in response to treatment in all protected animals, irrespective of treatment onset time. Non-stimulated pMCAO control group data demonstrate that reperfusion is not spontaneous. This makes the complete protection observed in the majority of animals stimulated at 2 hours post-pMCAO even more surprising as these animals recovered despite having been in this severely ischemic state for two full hours. In summary, when delivered within a 2 hour window post- pMCAO, whisker stimulation treatment initiated reperfusion and a gradual recovery of cortical function that was completed or nearly completed within the treatment period.