Treadmill Intervention Attenuates the Cafeteria Diet-Induced Impairment of Stress-Coping Strategies in Young Adult Female Rats.
Treadmill Intervention Attenuates the Cafeteria Diet-Induced Impairment of Stress-Coping Strategies in Young Adult Female Rats.
复制标题
DOI：
10.1371/journal.pone.0153687
复制
发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Escorihuela RM
中科院分区：
文献类型：
--
作者：
Cigarroa I;Lalanza JF;Caimari A;del Bas JM;Capdevila L;Arola L;Escorihuela RM
目前，青少年和成年人中因饮食引起的超重和肥胖的患病率正在持续增长。虽然肥胖的有害生化和代谢后果已经被广泛研究，但它对压力应对行为的影响以及它与特定运动剂量的相互作用(在强度、持续时间和频率方面)还需要进一步研究。为此，我们给青春期大鼠喂食肥胖饮食(自助餐厅饮食，CAF)或标准饮食(ST)。每组根据跑步机干预的类型分为四个亚组：不接受任何操作的静坐组；接触固定跑步机的对照组；以12m/min的速度训练的低强度跑步机组；以17m/min的速度训练的高强度跑步机组。饮食和跑步机干预从断奶开始，持续8周。在第7周，受试者在开放领域测试中进行焦虑样行为测试，并在双向主动回避范式中测试应对策略，并在第8周处死受试者进行生物测定和代谢特征分析。饲喂CAF可增加体重增加，增加相对腹膜后白色脂肪组织(RWAT%)，增加血糖、胰岛素、甘油三酯和瘦素水平，降低胰岛素敏感性。跑步机干预部分逆转了RWAT%和甘油三酯的变化；在更高的强度下，它降低了喂食CAF的动物的瘦素水平。在双向主动回避评估中，喂食CAF降低了运动活动，并损害了表现。跑步机干预减少了穿梭箱中的排便，这表明焦虑有所减轻。以17m/min的速度进食CAF结合跑步机训练增加了在开阔场地中心停留的时间，更重要的是，部分逆转了双向主动回避缺陷。总而言之，这项研究表明，在减少焦虑样行为的剂量下，跑步机运动在主动回避行为方面部分改善了CAF喂养的动物的应对策略。这种影响在较低剂量的跑步机训练中没有观察到。
The current prevalence of diet-induced overweight and obesity in adolescents and adults is continuously growing. Although the detrimental biochemical and metabolic consequences of obesity are widely studied, its impact on stress-coping behavior and its interaction with specific exercise doses (in terms of intensity, duration and frequency) need further investigation. To this aim, we fed adolescent rats either an obesogenic diet (cafeteria diet, CAF) or standard chow (ST). Each group was subdivided into four subgroups according to the type of treadmill intervention as follows: a sedentary group receiving no manipulation; a control group exposed to a stationary treadmill; a low-intensity treadmill group trained at 12 m/min; and a higher intensity treadmill group trained at 17 m/min. Both the diet and treadmill interventions started at weaning and lasted for 8 weeks. Subjects were tested for anxiety-like behavior in the open field test and for coping strategies in the two-way active avoidance paradigm at week 7 and were sacrificed at week 8 for biometric and metabolic characterization. CAF feeding increased the weight gain, relative retroperitoneal white adipose tissue (RWAT %), and plasma levels of glucose, insulin, triglycerides and leptin and decreased the insulin sensitivity. Treadmill intervention partially reversed the RWAT% and triglyceride alterations; at higher intensity, it decreased the leptin levels of CAF-fed animals. CAF feeding decreased the motor activity and impaired the performance in a two-way active avoidance assessment. Treadmill intervention reduced defecation in the shuttle box, suggesting diminished anxiety. CAF feeding combined with treadmill training at 17 m/min increased the time spent in the center of the open field and more importantly, partially reversed the two-way active avoidance deficit. In conclusion, this study demonstrates that at doses that decreased anxiety-like behavior, treadmill exercise partially improved the coping strategy in terms of active avoidance behavior in the CAF-fed animals. This effect was not observed at lower doses of treadmill training.