Gene expression analysis of the hedgehog signaling cascade in the chick midbrain and spinal cord
Gene expression analysis of the hedgehog signaling cascade in the chick midbrain and spinal cord
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DOI：
10.1002/dvdy.21146
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发表时间：
2007-05-01
影响因子：
2.5
通讯作者：
Agarwala, Seema
中科院分区：
文献类型：
--
作者：
Aglyamova, Galina V.;Agarwala, Seema
信号分子Sonic Hedgehog（SHH）在脊椎动物腹侧中脑的形成中起着关键作用。我们最近的研究已经确定，刺猬（HH）信号在鸡中脑的要求是调制空间和时间在整个中脑原基的复杂方式。不幸的是，目前尚不清楚可能调节中脑中HH信号的下游调节因子的表达模式。为了填补这一空白，我们研究了在中脑和脊髓中HH信号级联中起作用的14个基因的表达模式。我们的结果表明，SHH在中轴中内胚层中的表达开始于已知HH受体/HH结合蛋白（例如，PTC 1、PTC 2、HHIP、BOC、MEGALIN）。在中脑，PTC和GLI基因表达，然后从腹侧中线很早就消除了。然而，它们在限定SHH源的中脑区域中表现出高的和持续的表达。有趣的是，多种HH结合蛋白（BOC，MEGALIN）和HH效应物（GLI 1 -3，SMO，SUFU，DZIP）在背侧中脑和中脑-后脑边界中表达。最后，我们首次报道了IHH在脊髓的中间区域表达，其表达与SHH不重叠。发展动力学236：1363-1373，2007年。(C)2007 Wiley-Liss，Inc.
The signaling molecule Sonic Hedgehog (SHH) plays a critical role in patterning the ventral midbrain of vertebrates. Our recent studies have established that the requirement for Hedgehog (HH) signaling in the chick midbrain is modulated spatially and temporally in a complex manner across the midbrain anlage. Unfortunately, the patterns of expression of downstream regulators that might modulate the HH signal in the midbrain are not currently known. To fill this gap, we have examined across time, the expression pattern of 14 genes that function in the HH signaling cascade in the midbrain and spinal cord. Our results suggest that SHH expression in the axial mesendoderm begins before the expression of known HH receptors/HH-binding proteins (e.g., PTC1, PTC2, HHIP, BOC, MEGALIN). In the midbrain, PTC and GLI genes are expressed and then eliminated very early from the ventral midline. However, they exhibit high and persistent expression in the midbrain region circumscribing the SHH source. Intriguingly, multiple HH-binding proteins (BOC, MEGALIN) and HH effectors (GLI1-3, SMO, SUFU, DZIP) are expressed in the dorsal midbrain and the midbrain-hindbrain boundary. Finally, we report for the first time that IHH is expressed in intermediate regions of the spinal cord, where its expression does not overlap with that of SHH. Developmental Dynamics 236:1363-1373, 2007. (C) 2007 Wiley-Liss, Inc.