The rate of cell growth is governed by cell cycle stage
The rate of cell growth is governed by cell cycle stage
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DOI：
10.1101/gad.1777309
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发表时间：
2009-06-15
影响因子：
10.5
通讯作者：
Amon, Angelika
中科院分区：
文献类型：
--
作者：
Goranov, Alexi I.;Cook, Michael;Amon, Angelika
细胞生长是细胞周期进程的基本要求。虽然人们通常认为生长与细胞周期位置无关，但这种关系尚未得到仔细研究。这里我们展示了在出芽酵母中，细胞生长的能力在细胞周期中发生了变化。我们发现细胞生长在后期和G1比在其他细胞周期阶段更快。我们证明了极化肌动蛋白细胞骨架的建立——无论是作为正常细胞分裂的结果，还是通过激活交配信息素反应——都能有效地减弱蛋白质的合成和生长。我们进一步通过群体和单细胞分析表明，生长在一个不受干扰的细胞周期中变化，在极化生长时减慢。我们的研究揭示了细胞周期位置调节生长的基本关系。
Cell growth is an essential requirement for cell cycle progression. While it is often held that growth is independent of cell cycle position, this relationship has not been closely scrutinized. Here we show that in budding yeast, the ability of cells to grow changes during the cell cycle. We find that cell growth is faster in cells arrested in anaphase and G1 than in other cell cycle stages. We demonstrate that the establishment of a polarized actin cytoskeleton-either as a consequence of normal cell division or through activation of the mating pheromone response-potently attenuates protein synthesis and growth. We furthermore show by population and single-cell analysis that growth varies during an unperturbed cell cycle, slowing at the time of polarized growth. Our study uncovers a fundamental relationship whereby cell cycle position regulates growth.