NUCLEOTIDE-SEQUENCE ANALYSIS OF THE STRUCTURAL GENES FOR SHIGA-LIKE TOXIN-1 ENCODED BY BACTERIOPHAGE-933J FROM ESCHERICHIA-COLI
NUCLEOTIDE-SEQUENCE ANALYSIS OF THE STRUCTURAL GENES FOR SHIGA-LIKE TOXIN-1 ENCODED BY BACTERIOPHAGE-933J FROM ESCHERICHIA-COLI
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DOI：
10.1016/0882-4010(87)90106-9
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发表时间：
1987-02-01
影响因子：
3.8
通讯作者：
OBRIEN, AD
中科院分区：
文献类型：
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作者：
JACKSON, MP;NEWLAND, JW;OBRIEN, AD
对以前从大肠杆菌噬菌体933 J中克隆的志贺样毒素I基因进行了核苷酸序列分析。发现两个结构基因slt-IA和slt-I B定位在一个转录单位上，slt-IA位于slt-I B之前。12个碱基对的缺口将slt-IA和slt-I B的同相开放阅读框分开。在slt-IA和slt-I B基因的5“处鉴定了推定的核糖体结合位点。SLT-1的核苷酸序列的翻译揭示了A和B亚基都是由信号肽合成的。成熟A亚基的计算分子量为32，211，而成熟B亚基的分子量为7690。
A nucleotide sequence analysis was performed on the Shiga-like toxin I genes previously cloned from the Escherichia coli bacteriophage 933J. Two structural genes designed slt-I A and slt-I B were found to be oriented on a single transcriptional unit with slt-I A preceding slt-I B. A 12 base pair gap separated the in-phase open reading frames of slt-I A and slt-I B. Putative ribosome binding sites were identified 5'' to both the slt-I A and slt-I B genes. Translation of the SLT-I nucleotide sequence revealed that both the A and B subunits were synthesized with signal peptides. The molecular weight calculated for the mature A subunit was 32,211 while the molecular weight of the mature B subunit was 7690.