Droplet-based microfluidic system for multicellular tumor spheroid formation and anticancer drug testing
Droplet-based microfluidic system for multicellular tumor spheroid formation and anticancer drug testing
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DOI：
10.1039/c004590j
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发表时间：
2010-01-01
期刊：
影响因子：
6.1
通讯作者：
Cheung, Karen C.
中科院分区：
文献类型：
--
作者：
Yu, Linfen;Chen, Michael C. W.;Cheung, Karen C.
创建多细胞肿瘤球体对于表征抗癌治疗至关重要，因为它可能提供比肿瘤细胞单层培养更好的模型。此外，持续的动态灌流允许建立长期的细胞培养和随后的多细胞球体形成。一种基于液滴的微流控系统被用来与包埋的乳腺肿瘤细胞形成藻酸盐微珠。凝胶后，海藻酸盐珠被困在微筛结构中，在连续灌流系统中进行细胞培养。藻酸盐环境允许细胞增殖和多细胞球体的形成。与传统的单层培养相比，肿瘤球体对阿霉素和抗癌药物的剂量依赖性反应显示出多细胞耐药。在设备种植过程、细胞增殖和球体形成、药物治疗和成像过程中，微筛结构保持每个珠子在相同位置的恒定位置，允许进行时间和空间跟踪。
Creating multicellular tumor spheroids is critical for characterizing anticancer treatments since it may provide a better model than monolayer culture of tumor cells. Moreover, continuous dynamic perfusion allows the establishment of long term cell culture and subsequent multicellular spheroid formation. A droplet-based microfluidic system was used to form alginate beads with entrapped breast tumor cells. After gelation, the alginate beads were trapped in microsieve structures for cell culture in a continuous perfusion system. The alginate environment permitted cell proliferation and the formation of multicellular spheroids was observed. The dose-dependent response of the tumor spheroids to doxorubicin, and anticancer drug, showed multicellular resistance compared to conventional monolayer culture. The microsieve structures maintain constant location of each bead in the same position throughout the device seeding process, cell proliferation and spheroid formation, treatment with drug, and imaging, permitting temporal and spatial tracking.