Probability paper analysis of flow cytofluorometric measurements of cellular deoxyribonucleic acid content.
Probability paper analysis of flow cytofluorometric measurements of cellular deoxyribonucleic acid content.
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细胞脱氧核糖核酸含量的流式细胞荧光测量的概率纸分析。
DOI：
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发表时间：
1981
影响因子：
3.2
通讯作者：
J. Combs
中科院分区：
文献类型：
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作者：
J. E. Neely;J. Combs
描述了通过流式细胞荧光测定法获得的细胞脱氧核糖核酸 (DNA) 直方图的图形分析。该技术利用概率纸以累积百分位形式绘制数据图表。从该图形表示中，可以分离出对直方图有贡献的各个正态分布并进行统计描述。 G0G1 和 G2M 分布是独立描述的，S 期符合分析结果所示的 1--5 重叠正态分布。该技术具有多种优点，包括相对简单的数学计算以及对分析的假设或限制很少。事实证明，它可用于分析增殖和非增殖群体以及具有异常发现（例如碎片或异常 DNA 含量）的分布。
A graphic analysis of cellular deoxyribonucleic acid (DNA) histograms obtained by flow cytofluorometry is described. This technique utilizes probability paper that graphs data in cumulative percentile form. From this graphic representation, individual normal distributions contributing to the histogram may be isolated and statistically describe. G0G1 and G2M distributions are independently described and S phase is fit to 1--5 overlapping normal distributions as dictated by the analysis results. This technique offers several advantages, including relatively simple mathematical calculations and few assumptions or restraints placed on the analysis. It has proved to be useful in analyzing proliferating and nonproliferating populations as well as distributions with unusual findings such as debris or aberrant DNA contents.