Association of centrosomal kinase STK15/BTAK mRNA expression with chromosomal instability in human breast cancers
Association of centrosomal kinase STK15/BTAK mRNA expression with chromosomal instability in human breast cancers
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DOI：
10.1002/ijc.1200
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发表时间：
2001-05-01
影响因子：
6.4
通讯作者：
Noguchi, S
中科院分区：
文献类型：
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作者：
Miyoshi, Y;Iwao, K;Noguchi, S
中心体丝氨酸/苏氨酸激酶 STK15/BTAK 的过度表达会诱导中心体扩增，从而导致细胞培养中的染色体不稳定 (CIN)。在本研究中，我们研究了人类乳腺癌中 STK15/BTAK mRNA 表达与 CIN 和各种临床病理因素的相关性。 STK15/BTAK mRNA 水平通过实时 PCR 进行定量，CIN 值通过使用着丝粒探针对 1、11 和 17 号染色体进行 FISH 分析来确定。乳腺癌中的 STK15/BTAK mRNA 水平（0.310 +/- 0.413，平均值 +/- SD，n = 47）显着（p < 0.01）高于正常乳腺组织（0.044 0.029，n = 9），此外，根据 STK15/STAK mRNA 表达水平将乳腺癌分为 3 组（低、中和高）。低表达组（27.9 ± 12.6%，n = 18）的CIN值显着（p < 0.01）高于正常乳腺组织（9.2 ± 2.6%，n = 6），高表达组的CIN值（38.0 ± 12.7%，n = 14）显着（p < 0.05）高于低表达组。 STK15/BTAK mRNA 表达与高组织学分级以及雌激素和孕激素受体阴性呈显着（p < 0.05）相关。我们的结果表明，STK15/BTAK mRNA 在大多数乳腺癌中过表达，并且其过表达与 GIN 显着相关，表明 STK15/BTAK 通过诱导 GIN 参与癌发生。 STK15/BTAK mRNA 水平可能可作为不良预后和内分泌治疗耐药性的指标。 (C) 2001 年威利利斯。公司
Over-expression of a centrosomal serine/threonine kinase, STK15/BTAK induces centrosome amplification, which results in chromosomal instability (CIN) in cell culture. In the present study, we investigated the correlation of STK15/BTAK mRNA expression with CIN and various clinicopathological factors in human breast cancer. STK15/BTAK mRNA levels were quantified by real-time PCR, and CIN values were determined by FISH analysis of chromosomes 1, 11 and 17 using centromeric probes. STK15/BTAK mRNA levels (0.310 +/- 0.413, mean +/- SD, n = 47) in breast cancers were significantly (p < 0.01) higher than those in normal breast tissues (0.044 0.029, n = 9), Furthermore, breast cancers were divided into 3 groups (low, intermediate and high) according to STK15/STAK mRNA expression levels. CIN values of the low-expression group (27.9 +/- 12.6%, n = 18) were significantly (p < 0.01) higher than those of normal breast tissues (9.2 2.6%, n = 6), and those of the high-expression group (38.0 +/- 12.7%, n = 14) were significantly (p < 0.05) higher than those of the low-expression group. STK15/BTAK mRNA expression showed a significant (p < 0.05) correlation with high histological grade and negativity of estrogen and progesterone receptors, Our results demonstrate that STK15/BTAK mRNA is over-expressed in the majority of breast cancers and its over-expression is significantly associated with GIN, implicating STK15/BTAK in carcinogenesis through induction of GIN. STK15/BTAK mRNA levels might be useful as an indicator of poor prognosis and resistance to endocrine therapy. (C) 2001 Wiley-Liss. Inc.