In vitro biological assessment of Berberis vulgaris and its active constituent, berberine: antioxidants, anti-acetylcholinesterase, anti-diabetic and anticancer effects.
In vitro biological assessment of Berberis vulgaris and its active constituent, berberine: antioxidants, anti-acetylcholinesterase, anti-diabetic and anticancer effects.
复制标题
对Berberis ulgaris及其活性成分的体外生物学评估：抗氧化剂，抗乙酰胆碱酯酶，抗糖尿病和抗癌作用。
DOI：
10.1186/1472-6882-13-218
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发表时间：
2013-09-05
影响因子：
--
通讯作者：
El Demellawy MA
中科院分区：
文献类型：
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作者：
Abd El-Wahab AE;Ghareeb DA;Sarhan EE;Abu-Serie MM;El Demellawy MA
小檗（Berberis vulgaris）是一种有着传统草药历史的知名植物。对小檗粗提物和小檗碱活性化合物的体外生物活性（抗氧化、胆碱能、降糖和抗癌）进行生物筛选和比较。采用分光光度法测定了枸杞提取物和氯化小檗碱对细胞硫代巴比妥酸活性物质（TBARS）形成、二苯- α-苦酰肼（DPPH）氧化、细胞一氧化氮（NO）自由基清除能力、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）、乙酰胆碱酯酶（AChE）和α-葡萄糖苷酶活性的影响。另一方面，用中性红色摄取法与对照正常细胞（PBMC）比较，评价了提取物和小檗碱对MCF-7、HepG-2和Caco-2三种不同细胞系的抗癌作用。结果表明，小檗粗提物中小檗碱含量为0.6 mg /mg。杨梅提取物通过降低TBARS、NO和与GPx和SOD过度激活相关的DPPH的氧化，显示出强大的抗氧化能力。小檗粗提物对α-葡萄糖苷酶的抑制作用强于氯化小檗碱，二者对AChE的抑制作用相同。此外，不同浓度的氯化小檗碱和小檗乙醇提取物对正常血细胞（PBMC）均无生长抑制作用。另外，小檗碱和小檗醇提物对乳腺癌、肝癌和结肠癌细胞株（分别为MCF7、HepG2和cco -2）在24 ~ 72小时的不同孵育时间均有抑制作用，且抑制作用随时间增加呈剂量依赖性。这项工作证明了小檗粗提物及其活性生物碱小檗碱在抑制脂质过氧化方面的潜力，表明其在治疗肝脏氧化应激、阿尔茨海默病和特发性男性因素不育症方面的应用前景广阔。此外，小檗乙醇提取物是安全无毒的提取物，不抑制PBMC的生长，诱导癌细胞死亡，可以恢复其强大的抗氧化活性。
Berberis vulgaris is a well known plant with traditional herbal medical history. The aims of this study was to bioscreen and compare the in vitro biological activity (antioxidant, cholinergic, antidaibetic and the anticancer) of barberry crude extract and berberine active compound. The effect of B. vulgaris extract and berberine chloride on cellular thiobarbituric acid reactive species (TBARS) formation, diphenyle–α-picrylhydrazyl (DPPH) oxidation, cellular nitric oxide (NO) radical scavenging capability, superoxide dismutase (SOD), glutathione peroxidase (GPx), acetylcholinesterase (AChE) and α-gulcosidase activities were spectrophotometrically determined. On the other hand, the effect of extract and berberine as anticancer was estimated on three different cell lines which were MCF-7, HepG-2, and Caco-2 cells by using neutral red uptake assay which compared with control normal cells (PBMC). Our results showed that barberry crude extract contains 0.6 mg berberine/mg crude extract. Barberry extract showed potent antioxidative capacity through decreasing TBARS, NO and the oxidation of DPPH that associated with GPx and SOD hyperactivation. Inhibitory effect of berberis crude extract on α-glucosidase was more potent than that of berberine chloride, while both had the same AChE inhibitory effect. Besides, different concentrations of both berberine chloride and barberry ethanolic extract showed to have no growth inhibitory effect on normal blood cells (PBMC). Otherwise, both berberine chloride and barberry ethanolic extract showed to have inhibitory effect on the growth of breast, liver and colon cancer cell lines (MCF7, HepG2 and CACO-2, respectively) at different incubation times starting from 24 hrs up to 72 hrs and the inhibitory effect increased with time in a dose dependant manner. This work demonstrates the potential of the barberry crude extract and its active alkaloid, berberine, on suppressing lipid peroxidation, suggesting a promising use in the treatment of hepatic oxidative stress, Alzheimer and idiopathic male factor infertility. Beside, berberis vulgaris ethanolic extract is safe non-toxic extract as it was not inhibit the growth of PBMC that can induce cancer cell death that could return to its powerful antioxidant activity.