The inner centromere protein (INCENP) antigens: movement from inner centromere to midbody during mitosis.
The inner centromere protein (INCENP) antigens: movement from inner centromere to midbody during mitosis.
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DOI：
10.1083/jcb.105.5.2053
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发表时间：
1987-11
期刊：
影响因子：
--
通讯作者：
Earnshaw WC
中科院分区：
文献类型：
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作者：
Cooke CA;Heck MM;Earnshaw WC
我们描述了一组新的多肽抗原，其在有丝分裂期间结构定位发生显著变化。在中期，这些抗原界定了一种位于姐妹染色单体之间的新的染色体亚结构。由于这些抗原集中在着丝粒周围区域，我们暂时将它们命名为内着丝粒蛋白（INCENPs）。内着丝粒蛋白（两种分子量分别为155和135kD的多肽）是通过一种针对有丝分裂染色体支架组分的大量蛋白质所产生的单克隆抗体鉴定出来的。这两种多肽是已知结合最紧密的染色体蛋白质。当制备支架时，100%可检测到的内着丝粒蛋白仍与支架相关联。因此，我们对后期内着丝粒蛋白的命运毫无准备。随着姐妹染色单体分离，内着丝粒蛋白完全从它们上面解离，在染色单体向纺锤体极迁移时留在赤道板处。在后期，内着丝粒蛋白存在于中央纺锤体的粗纤维上，并且在形成收缩环的区域与细胞膜紧密相邻。在末期，内着丝粒蛋白逐渐集中在形成的中间体上，并最终与之一同被丢弃。这些数据提示了内着丝粒蛋白在体内的几种可能作用：姐妹染色单体配对的调节、分裂平面的稳定以及后期纺锤体极的分离。
We describe a novel set of polypeptide antigens that shows a dramatic change in structural localization during mitosis. Through metaphase these antigens define a new chromosomal substructure that is located between the sister chromatids. Because the antigens are concentrated in the pericentromeric region, we have provisionally termed them the INCENPs (inner centromere proteins). The INCENPs (two polypeptides of 155 and 135 kD) were identified with a monoclonal antibody that was raised against the bulk proteins of the mitotic chromosome scaffold fraction. These two polypeptides are the most tightly bound chromosomal proteins known. When scaffolds are prepared, 100% of the detectable INCENPs remain scaffold associated. We were therefore unprepared for the fate of the INCENPs at anaphase. As the sister chromatids separate, the INCENPs dissociate fully from them, remaining behind at the metaphase plate as the chromatids migrate to the spindle poles. During anaphase the INCENPs are found on coarse fibers in the central spindle, and also in close apposition to the cell membrane in the region of the forming contractile ring. During telophase, the INCENPs gradually become focused onto the forming midbody, together with which they are ultimately discarded. Several possible in vivo roles for the INCENPs are suggested by these data: regulation of sister chromatid pairing, stabilization of the plane of cleavage, and separation of spindle poles at anaphase.