Fast and accurate detection of action potentials from somatic calcium fluctuations
Fast and accurate detection of action potentials from somatic calcium fluctuations
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DOI：
10.1152/jn.00084.2008
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发表时间：
2008-09-01
影响因子：
2.5
通讯作者：
Ikegaya, Yuji
中科院分区：
文献类型：
--
作者：
Sasaki, Takuya;Takahashi, Naoya;Ikegaya, Yuji
从神经元群体中进行大规模记录是研究复杂脑功能的一种有前途的策略。利用动作电位可靠地引起细胞体中瞬时钙波动的事实，功能性多神经元钙成像（fMCI）监测数百个神经元的阈上活动。然而，fMCI的局限性在于其从体细胞钙波动中提取峰电位的半手动过程，这不仅耗时而且与人为错误相关。在这里，我们描述了一种新的自动方法，结合主成分分析和支持向量机。这种简单的算法比人类操作员更快速可靠地确定钙荧光痕迹中尖峰的时间。
Large-scale recording from a population of neurons is a promising strategy for approaching the study of complex brain functions. Taking advantage of the fact that action potentials reliably evoke transient calcium fluctuations in the cell body, functional multineuron calcium imaging (fMCI) monitors the suprathreshold activity of hundreds of neurons. However, a limitation of fMCI is its semi-manual procedure of spike extraction from somatic calcium fluctuations, which is not only time consuming but is also associated with human errors. Here we describe a novel automatic method that combines principal-component analysis and support vector machine. This simple algorithm determines the timings of the spikes in calcium fluorescence traces more rapidly and reliably than human operators.