Use of gain-of-function study to delineate the roles of crumbs in Drosophila eye development
Use of gain-of-function study to delineate the roles of crumbs in Drosophila eye development
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DOI：
10.1159/000073964
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发表时间：
2003-01-01
影响因子：
11
通讯作者：
Yang, VC
中科院分区：
文献类型：
--
作者：
Fan, SS;Chen, MS;Yang, VC
细胞极性基因crumbs（crb）参与果蝇视网膜的发育和退化。CRB 1（果蝇crb的人类同源物）的突变也会导致视网膜色素变性和莱伯先天性黑蒙。在这项研究中，我们使用功能获得的方法来描绘crb在果蝇眼睛发育中的作用。在三龄幼虫阶段，眼的发育开始与感光细胞分化和感光细胞核的定位在视网膜上皮的顶端细胞室。在蛹期，分化的光感受器开始在其顶端表面形成光敏结构，即杆粒。使用GMR-Gal 4驱动Crb蛋白在第三龄眼盘的过表达，我们发现光感受器的分化被破坏，并且分化的光感受器的核未能占据顶室。使用hs-Gal 4驱动蛹眼中Crb过表达导致干扰粘附连接的延伸和横纹肌的构建，并且这些缺陷是阶段依赖性的。这项功能获得性研究使我们能够描绘Crb在果蝇眼睛发育的选择性阶段的作用。版权所有（C）2003国家科学理事会，中华民国和S. Karger AG，巴塞尔。
The cell polarity gene, crumbs (crb), has been shown to participate in the development and degeneration of the Drosophila retina. Mutations in CRB1, the human homologue of Drosophila crb, also result in retinitis pigmentosa and Leber congential amaurosis. In this study, we used the gain-of-function approach to delineate the roles of crb in developing Drosophila eye. In the third-instar larval stage, eye development is initiated with photoreceptor differentiation and positioning of photoreceptor nuclei in the apical cellular compartment of retinal epithelium. In the pupal stage, differentiated photoreceptors begin to form the photosensitive structures, the rhabdomeres, at their apical surface. Using GMR-Gal4 to drive overexpression of the Crb protein at the third-instar eye disc, we found that differentiation of photoreceptors was disrupted and the nuclei of differentiated photoreceptors failed to occupy the apical compartment. Using hs-Gal4 to drive Crb overexpression in pupal eyes resulted in interference with extension of the adherens junctions and construction of the rhabdomeres, and these defects were stage-dependent. This gain-of-function study has enabled us to delineate the roles of Crb at selective stages of eye development in Drosophila. Copyright (C) 2003 National Science Council, ROC and S. Karger AG, Basel.