SCHISTOSOMA-JAPONICUM - IDENTIFICATION AND CHARACTERIZATION OF NEUTROPHIL CHEMOTACTIC FACTORS FROM EGG ANTIGEN
SCHISTOSOMA-JAPONICUM - IDENTIFICATION AND CHARACTERIZATION OF NEUTROPHIL CHEMOTACTIC FACTORS FROM EGG ANTIGEN
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DOI：
10.1016/0014-4894(85)90026-8
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发表时间：
1985-01-01
影响因子：
2.1
通讯作者：
ISHII, A
中科院分区：
文献类型：
--
作者：
OWHASHI, M;HORII, Y;ISHII, A
在日本血吸虫可溶性卵抗原制剂中鉴定出两种中性粒细胞趋化因子。通过凝胶过滤、阴离子交换层析、等电聚焦或亲和层析，无法将高分子量的中性粒细胞趋化因子与嗜酸性粒细胞趋化因子分离开来：该中性粒细胞趋化因子显然与我们先前从可溶性卵抗原中纯化的高分子量嗜酸性粒细胞趋化因子相同。嗜酸性趋化因子对中性粒细胞的趋化活性在高酸盐氧化下是稳定的，但受到加热或Pronase消化的显著影响，表明嗜酸性趋化因子的决定因素存在于嗜酸性趋化因子的肽段。通过凝胶过滤或阴离子交换层析可将低分子量中性粒细胞趋化因子与高分子量嗜酸性粒细胞趋化因子分离。该中性粒细胞趋化因子具有较好的疏水性和热稳定性，但对Pronase或carboxypeptidase A的消化很敏感。这些结果表明，这些化学引诱剂在中性粒细胞和嗜酸性粒细胞表面的受体可能是不同的。我们推测，日本血吸虫卵中嗜中性粒细胞趋化因子和嗜酸性粒细胞趋化因子是造成日本血吸虫卵周围嗜中性粒细胞和嗜酸性粒细胞聚集的原因。
Two neutrophil chemotactic factors were identified in soluble egg antigen preparations of Schistosoma japonicum. The higher-molecular-weight neutrophil chemotactic factor was not separable from eosinophil chemotactic factor by means of gel filtration, anion-exchange chromatography, isoelectric focusing, or affinity chromatography: this neutrophil chemotactic factor is apparently identical to the higher-molecular-weight eosinophil chemotactic factor which we purified previously from the soluble egg antigen. The chemotactic activity of the eosinophil chemotactic factor for neutrophils was stable to periodate oxidation but was notably affected by heating or Pronase digestion, suggesting that the determinant for neutrophil chemotaxis exists on the peptide moiety of the eosinophil chemotactic factor. The lower-molecular-weight neutrophil chemotactic factor was separable from the higher-molecular-weight eosinophil chemotactic factor by gel filtration or anion-exchange chromatography. This neutrophil chemotactic factor was rather hydrophobic and heat-stable, but was sensitive to Pronase or carboxypeptidase A digestion. These results suggest that the receptors on the surfaces of neutrophils and eosinophils for those chemoattractants would be different from each other. We suppose that neutrophil chemotactic factors and eosinophil chemotactic factors from the eggs are responsible for neutrophil and eosinophil accumulation around the eggs in schisotosomiasis japonica.